中文摘要：

目的：研究青蒿油制剂复尔康注射液对肝癌HepG2细胞增殖和凋亡的影响。方法：CCK8试剂盒（cell counting kit-8）检测复尔康注射液对肝癌HepG2细胞增殖的抑制作用；Hoechst33258荧光染色观察HepG2细胞凋亡的形态学改变；流式细胞仪AnnexinV／PI双染法检测HepG2细胞的凋亡率。结果：复尔康注射液可抑制HepG2细胞的增殖，IC50值为29．63±1．22μg／ml；复尔康注射液0．3125-10μg／ml作用48h后，Hoechst33258荧光染色均可见HepG2细胞出现核浓缩、核碎裂等细胞凋亡形态学改变，且呈现出一定的剂量依赖性；AnnexinV／PI双染法检测显示，不同浓度的复尔康注射液均可诱导HepG2细胞凋亡，凋亡率分别为4．71％±0．71％、10．05％±0．67％、15．08％±1．16％、23．59％±1．65％、32．30％±1．87％、42．09％±1．43％。结论：复尔康注射液可抑制HepG2细胞的增殖，诱导其凋亡。

英文摘要：

Objective： To study the effect of Fuerkang injection on the proliferation and apoptosis of hepatoblastoma G2 （HepG2） cell. Methods： The cytotoxicity of Fuerkang injection on HepG2 was determined by CCK8 （ cell counting kit-8 ） ; The cell apoptosis morphological changes was observed using Hoechst 33258 staining; The rate of apoptosis was detected by flow cytometry with Annexin-V/PI staining. Results： The Fuerkang injection can inhibit the proliferation of HepG2 cells, the IC50 value was 29.63±1. 22μg/ml; Typical morphological changes of cell apoptosis such as nuclear concertration and nuclear fragmentation were observed after treatment of Fuerkang injectiong at concentration of 0.3125 - 10μg/ml for 48 h in a dose-dependent manner; The apoptosis of HepG2 cells was induced by different concentration of Fuerkang injection and the apoptosis rate was 4.71% ± 0.71%, 10.05% ± 0.67%, 15.08% ± 1.16% ,23.59% ± 1.65% ,32.30% ± 1.87% and 42. 09% ± 1.43%, respectively . Conclusion： Fuerkang injection can inhibit the proliferation of HepG2 cells and induce their apeptosis.