中文摘要：

为了研究MADS-box家族转录调控因子GmNMHC5与植物激素分泌之间的关系,通过发根农杆菌介导下胚轴转化法获得根部过量表达GmNMHC5基因的嵌合体植株,采用GUS染色分析和GmNMHC5的相对表达量分析筛选阳性转基因毛状根。采用酶联免疫法测定V3期转基因毛状根和非转基因毛状根（对照）赤霉素和脱落酸含量,发现转基因毛状根部赤霉素的含量为6.65ng/g,比非转基因毛状根提高了18.5%;脱落酸含量为78.87 ng/g,比非转基因毛状根降低了19.6%。采用外源赤霉素（50μmol/L）和脱落酸（50μmo/L）处理植株根部,V3期根部GmNMHC5的相对表达量分别是对照组的4.52倍和0.31倍,说明外源赤霉素可以极显著地促进根部GmNMHC5的表达,外源脱落酸可以极显著地抑制根部GmNMHC5的表达。上述研究结果表明,自贡冬豆根部GmNMHC5的表达与植物激素的分泌存在交互调控作用,协同调控植株的生长发育。

英文摘要：

To explore the relationship between GmNMHC5, a member of MADS - box transcription factor fami- ly, and the production of phytohorrnones in the roots of soybean （ Glycine max ） cv. Zigongdongdou, GmNMHC5 was transferred to soybean plant by Agrobacterium rhizogenes, the positive transgenic hairy root were selected with GUS staining and relative expression level of GmNMHC5. The content of phytohormones in the transgenic and non -transgenic hairy root（CK） were detected using ELISA method at V3 stage. The results indicated that, in com- parison with control, gibberellin A3 （ GA3 ） increased 18.5% and ABA content decreased 19.6%, respectively. These indicated that the overexpression of GmNMHC5 promoted the production of GA3 and suppressed the produc- tion of ABA. After treatment with exogenous GA3 （50t~moL/L） and exogenous ABA （50lxmol/L） , real -time fluo- rescent quantitative PCR analysis showed relative expression of GmNMHC5 in root were 4.52 and 0.31 times of the control at V3 stage. It was suggested that the promotional effects of exogenous GA3 and suppressive effects of ABA on the expression of GmNMHC5 were both highly significant in the root of soybean. These data revealed an interactive regulation between the expression of GmNMHC5 and the production of phytohormones in soybean.