为明确内生真菌对醉马草根际土壤线虫群落特征的影响,于2014年6,8和10月,对带内生真菌和不带内生真菌醉马草根际土壤进行采样,利用淘洗-过筛-蔗糖离心法分离线虫,根据线虫形态学特征进行鉴定,结果表明,本研究共捕获土壤线虫11889条,分别隶属于线虫动物门2纲6目22科37属,带内生真菌醉马草根际土壤线虫个体密度高于不带内生真菌醉马草,而类群数、多样性指数(H′)、均匀度指数(J)和丰富度指数(SR)均低于不带内生真菌醉马草,但处理间差异不显著,表明尽管内生真菌使醉马草根际土壤线虫个体密度有所增加,使类群数有所降低,但是内生真菌的存在对醉马草土壤线虫多样性没有影响;与不带内生真菌醉马草相比,带内生真菌醉马草根际土壤线虫MI指数显著升高,而PPI指数和PPI/MI值显著降低,表明内生真菌在一定程度上确实改变了土壤线虫功能类群组成,其中主要影响植物寄生线虫。
The aim of this study was to determine the effect of the Epichloёendophyte on soil nematodes and their community characteristics in the rhizosphere of Achnatherum inebrians.Soil nematodes were isolated and identified from the rhizosphere of A.inebrians plants grown with(E+)or without(E-)the Epichloёendophyte.A total of 11889 soil nematodes were collected,belonging to 37 genera,22families,six orders,and two classes.The individual density of soil nematodes was higher in soil of E+ plants than in soil of E- plants.The generic number,Shannon-Wiener index,Pielou index,and Margalef index were lower in E+soil than in Esoil,whereas the diversity index did not differ significantly between the two treatments.The results suggested that the Epichloёendophyte had a limited effect on the diversity of soil nematodes of A.inebrians.The maturity index(MI)of soil nematodes was significantly higher in the E+soil than in the E-soil,whereas the plant parasite index(PPI)and PPI/MI were significantly lower in the E+soil than in the E-soil.The presence of the Epichloёendophyte had a significant effect on MI,PPI,and PPI/MI.These results show that the Epichloёendophyte can change the composition of functional groups of soil nematodes,especially that of plantparasitic nematodes.