中文摘要：

目的观察糖肾方对2型糖尿病大鼠肝脏氧化应激水平及病理改变的影响,并探讨其可能的机制。方法采用高脂饲料喂养和小剂量链脲佐菌素（STZ）腹腔内注射方法,建立2型糖尿病合并非酒精性脂肪肝动物模型。将造模成功的大鼠随机分为正常组、模型组、糖肾方组和二甲双胍组,比较各组大鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、三酰甘油（TG）、总胆固醇（CHO）和低密度脂蛋白胆固醇（LDL-C）水平,肝组织超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、维生素E（VE）、一氧化氮（NO）和一氧化氮合酶（NOS）水平,并在光镜下观察各组大鼠肝组织病理形态学改变。结果与模型组相比,二甲双胍组和糖肾方组大鼠表现状态较好,毛色较光泽,粪便成形;二甲双胍组和糖肾方组大鼠的肝脏质量和肝脏质量指数较模型组明显下降,2组大鼠血清ALT、AST、TG、CHO、LDL-C水平显著降低。与模型组相比,糖肾方组大鼠肝组织匀浆中SOD、CAT、VE水平明显升高,NO、NOS水平减低;二甲双胍组大鼠肝组织匀浆中SOD、CAT水平明显升高,NO、NOS水平减低。HE染色显示二甲双胍组和糖肾方组较模型组大鼠肝细胞脂肪变性明显减少,肝细胞排列基本规则。结论糖肾方可改善2型糖尿病大鼠肝功能,降低血脂水平,减轻肝脏脂质沉积,其作用机制可能与其清除脂质过氧化物,改善肝组织氧化应激水平有关。

英文摘要：

Objective To investigate the effects of Tangshen Formula on liver oxidative stress in diabetic rats, andtheir mechanisms thereof.Methods Rat model of type 2 diabetes mellitus and nonalcoholic fatty liver disease was estab-lished by intraperitoneally injecting small dose of chain urea with cephalosporins（STZ） and feeding high fat fodder.Themodel rats were randomly divided into control group, model group, Tangshen Formula group and metformin group.The se-rum levels of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, triglyceride（TG）, cholesterol（CHO）, lowdensity lipoprotein-cholesterol（LDL-C）, and superoxide dismutase（SOD）, catalase（CAT）, vitamin E（VE）, nitric oxide（NO）and nitric oxide synthase（NOS） in liver were compared between four groups. Changes of pathological morphology were ob-served under light microscopy. Results There were significantly decrease in serum levels of ALT, AST, TG, CHO, LDL-Cin Tangshen Formula group and metformin group compared with those of model group. There were significantly higher levelsof SOD, CAT and VE, and lower levels of NO and NOS of liver homogenate in Tangshen Formula group than those of modelgroup. There were higher levels of SOD and CAT, and lower levels of NO and NOS in liver homogenate in metformin groupthan those of model group. HE staining showed that liver fatty degeneration was significantly reduced in metformin group andTangshen Formula group compared with that of model group. Conclusion The fatty liver in type 2 diabetic rats is signifi-cantly improved by Tangshen Formula treatment, which is probably associated with the regulation of the response level to oxi-dative stress in liver.