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柴黄益肾颗粒对糖尿病并发肝损伤大鼠TGF-β1及GLUT-1的调控作用研究
  • ISSN号:1001-0025
  • 期刊名称:中日友好医院学报
  • 时间:2012.12.15
  • 页码:348-352+386
  • 分类:R285.5[医药卫生—中药学;医药卫生—中医学]
  • 作者机构:[1]北京中医药大学,北京100029, [2]中日友好医院临床医学研究所,北京100029, [3]河北联合大学,唐山063000
  • 相关基金:国家自然科学基金(No.30973911,No.81173422); 国家国际科技合作专项(No.2011DFA31860)
  • 相关项目:糖肾方通过sEH/EETs调节NF-κB炎症通路治疗糖尿病肾病机制研究
中文摘要:

目的:观察柴黄益肾颗粒对糖尿病肾病并发肝损伤大鼠的肝保护作用,并探讨其可能机制。方法:单侧肾切除合并链脲佐菌素注射诱导糖尿病肾病大鼠模型,给药20周后,检测大鼠血糖、肝功能、肝组织病理、肝组织Ⅰ、Ⅲ型胶原及肝糖原蓄积程度,并检测肝组织葡萄糖转运蛋白-1(GLUT-1)、转化生长因子-β1(TGF-β1)mRNA及蛋白表达水平。结果:与假手术对照组比较,模型组大鼠肝组织表现为肝细胞索排列紊乱,炎性细胞浸润,糖原颗粒蓄积,Ⅰ、Ⅲ型胶原纤维异常增生,柴黄益肾颗粒组大鼠肝组织炎性细胞浸润、糖原蓄积程度及Ⅰ、Ⅲ型胶原纤维增生程度显著减轻,肝组织GLUT-1及TGF-β1 mRNA、TGF-β1蛋白表达显著低于模型组(P〈0.05)。结论:柴黄益肾颗粒对糖尿病肾病并发肝损伤大鼠具有肝保护作用,这可能与柴黄益肾颗粒显著降低肝组织GLUT-1及TGF-β1表达有关。

英文摘要:

Objective:To investigate the effect of Chaihuangyishen granule (CHYS)the expressions of glucose transporter-1 (GLUT-1)and transforming growth factor β1 (TGF-β1)in the liver of diabetic nephropathy rats, and probe the mechanism.Methods:Male Wistar rats were randomly divided into 12 rats in sham control group and 100 rats in uninephrectomy groups which were induced into diabetic model by injection of 40mg/kg STZ.The diabetic rats were then randomly divided into model group,CHYS low dosage group,CHYS medial dosage group,CHYS high dosage group and Fosinopril-treated group.The body weight,plasma glucose were measured.The liver pathomorphology was observed by HE staining and PAS staining, and type I and III collagen were measured with immunohistochemical technique.Liver GLUT-1 and TGF-β1 expression were mea- sured with reverse transeriptase-polymerase chain reaction (RT-PCR) and Western blot.Results:The glycogen accumulation and fibration were alleviated in the liver tissues of rats treated by CHYS,accompanying the sup- pression of type I and m collagen expression in the liver.Liver expression of GLUT-1 and TGF-β1 de- creased significantly compared with the model group(P〈0.05).Conclusions:CHYS have protective effect on ex- perimental liver injuries in diabetic nephropathy rats.The mechanism might be related to the suppression of expression of GLUT-1 and TGF-β1.

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期刊信息
  • 《中日友好医院学报》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国国家卫生和计划生育委员会
  • 主办单位:中日友好医院
  • 主编:柯元南
  • 地址:北京和平街北口樱花园东街2号中日友好医院内
  • 邮编:100029
  • 邮箱:zryhyyxb@yahoo.com.cn
  • 电话:010-84205632
  • 国际标准刊号:ISSN:1001-0025
  • 国内统一刊号:ISSN:11-2622/R
  • 邮发代号:82-458
  • 获奖情况:
  • 国内外数据库收录:
  • 被引量:8050