中文摘要：

对阳离子交换树脂CM52纯化类人胶原蛋白U（Human-like Collagen Bioprotein Ⅱ，HCB Ⅱ）的合适条件进行了研究。通过实验，确定了批量层析的最佳操作条件，即在pH=4．0、NaCl0．15mol／L、进料质量浓度7mg／mL、处理量17．26mL／g进行吸附，吸附时间为60min，然后在pH=4．0、NaCl0．30mol／L下进行脱附。实验表明：采用批量层析吸附HCBⅡ后上柱洗脱，树脂CM52对HCBⅡ的吸附量可达到48．6mg／g，回收率83．8％，最终纯化的HCBⅡ达电泳纯，相对分子质量为97k。

英文摘要：

The appropriate conditions for the purification of recombinant human collagen-like bioprotein Ⅱ （ HCB Ⅱ） by CM52 cation exchange were investigated. The experiment showed that the optimal operation conditions： HCB Ⅱ was absorbed for 60 min at pH =4.0, NaCl concentration of 0.15mol/L, crude protein mass concentration of 7 mg/mL, processing capacity of 17.26 mL/g by batch operation of chromatography, and the elution of HCB Ⅱ was done at pH = 4.0, the NaCl concentration of 0.30mol/L. The result shows that the capacity of adsorption of CM52 cation exchanger for HCB ii can reach 48.6 mg/g by batch operation; the total processing time is shorten and resolution is high, the recovery of aim protein HCB Ⅱ with a relative molecular mass of 97k is above 83.8% and the purity of HCB Ⅱ can reach electrophoresis grade purity.