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中文摘要：
目的 优化类人胶原蛋白基因工程茵高温诱导后的比生长速率。方法 通过分析不同比生长速率下发酵液中菌体浓度、类人胶原蛋白浓度、细胞得率系数（Yx/s）、产物得率系数（Yp／s）的变化，确定诱导后的最佳比生长速率。结果 当比生长速率为0．04～0．05h^-1，细胞浓度和类人胶原蛋白的浓度分别可达69．5g／L（DCW）和13．8g／L。结论 诱导后的比生长速率对细胞生长、类人胶原蛋白的合成均产生显著性影响。
英文摘要：
To optimize the specific growth rate of recombinant Escherichia coli BL expressing human-like collagen after temperature induction. Methods The key parameters, such as biomass, concentration of human-like collagen, cell yield coefficient （ Yx/s ） and product yield coefficient （ Yp/s ）, were analyzed and investigated to optimize the optimal specific growth rates. Results When the specific growth rate was controlled at 0. 04-0. 05 h^-1, the cell concentration and the concentration of human-like collagen could reach 69. 5 g/L （DCW） and 13.8 g/L respectively. Conclusion The specific growth rate of recombinant Escherichia coli BL after induction would affect cell growth and human-like collagen＇s synthesis obviously.
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