中文摘要：

用阳离子交换树脂CM-52分离纯化类人胶原蛋白Ⅱ（Human Collagen-like Bioprotein Ⅱ，HCBⅡ）。通过实验，分别确定了静态吸附和柱层析吸附的操作条件，即静态吸附：在pH4．0、NaCl0．15mol／L、进料浓度7g／L，处理量17．26ml／g树脂的条件下进行吸附，吸附时间为60min；柱层析：在pH4．0、NaCl0．15mol／L、进料浓度5g／L、流速5ml／min的条件下进行吸附。然后上柱，在pH4．0、NaCl0．30mol／L下进行脱附。实验表明，采用静态吸附的方式吸附HCBⅡ，然后上柱洗脱，HCBⅡ的吸附量可达到48、6mg／g树脂，回收率83．8％，操作时间短，分辨率高，最终纯化的HCBⅡ达电泳纯，分子量为97kD。

英文摘要：

A purification method of recombinant human collagen-like Bioprotein Ⅱ （HCB Ⅱ） by the CM52 cation exchange chromatography were reported. The batch operation and normal chromatography column method were used in experiments, i.e., Batch operation of chromatography： HCB Ⅱ was absorbed under pH 4.0, NaCl （ionic strength） 0.15mol/L, 17.26ml （protein volume）/g （resin） and concentration of crude protein liquid 7g/L; Column chromatography： HCB Ⅱ was absorbed under pH 4.0, NaCl （ionic strength） 0.15mol/L, flow rate 5ml/min and concentration of crude protein liquid 5g/L, the elution of HCB Ⅱ was pH 4.0, the concentration of NaCl （ionic strength） 0.30mol/L. As a result, the CM52 cation exchange chromatography was of better capacity of adsorption for HCB Ⅱ and the elution was simple; the total purity process time would be shorten and resolution was high, The recovery of aim protein HCB Ⅱ with a molecular weight around 97kD was above 83.8% and purity of HCB Ⅱ could reach electrophoresis grade purity.