中文摘要：

在体外模拟人体消化环境,以考来烯胺作为阳性对照,采用分光光度法测定了体外儿茶素EGCG对6种胆酸盐的结合能力;同时探讨了EGCG对高血脂模型大鼠血脂代谢的调控功能。研究结果显示,除胆酸钠外,儿茶素EGCG与6种胆酸盐平均结合率均较高,达到60%以上;其中EGCG结合牛磺胆酸钠（STC）、甘氨胆酸钠（SGC）、牛磺石胆酸（EA）和牛磺脱氧胆酸（ST）的平均结合率分别是考来烯胺的78.99%、90.14%、82.00%和93.48%。高脂模型大鼠脂质代谢结果显示血清中TC、TG及LDL-C含量与模型组相比显著下降（P〈0.05）,HDL-C显著升高（P〈0.05）,肝脏组织HE染色显示EGCG处理组肝细胞脂肪变性明显改善,肝细胞排列整齐。因此,上述研究结果表明儿茶素调控脂质代谢的机制至少部分源自对小肠中胆汁酸的吸附,抑制其重吸收,从而发挥其脂质代谢调控功能。

英文摘要：

Basing on the simulation of human digestive environment in vitro and using the cholestyramine as a positive reagent,the binding capacity of catechin EGCG to six bile acid salts was detected by ultraviolet-visible（UV）spectrometer.At the same time,EGCG regulation of lipid metabolism in the hyperglycemia rats was also investigated.And the results showed that the binding rates of EGCG to six bile acid salts were all more than 60%,except for SC.Compared to cholestyramine,the relative rates of EGCG with STC,SGC,EA and ST were 78.99%,90.14%,82.00% and 93.48%,respectively.And EGCG regulation of lipid metabolism in the hyperglycemia rats revealed that serum TC,TG and LDL-C levels were significantly lower in EGCG-treated group than high-fat model group（P〈0.05）,while HDL-C levels were significantly higher（P〈0.05）.HE staining in rat liver showed that liver steatosis was attenuated significantly and hepatocyte ranged orderly.Therefore,all the above research results showed that regulation mechanism of lipid metabolism by catechins was at least partly attributed to their binding capacity to bile acid.So the bile acid reabsorption was prevented and exhibited the regulation function on the lipid metabolism.