中文摘要：

利用GC-FID建立了草鱼血清、肝胰脏、小肠及肝细胞培养液中邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸丁基苄酯（BBP）和邻苯二甲酸二乙基己基酯（DEHP）同时检测方法，并利用LC-MS／MS方法进行了DEP、DBP、BBP及DEHP在草鱼脏器中的代谢规律研究，以揭示邻苯二甲酸酯类（PhthalicAcidEsters，PAEs）化合物在草鱼体内的致毒机制。结果表明，DEP、DBP、BBP和DEHP在草鱼肝细胞中未见明显代谢，但在草鱼血清、肝胰脏匀浆液和小肠匀浆液中均出现不同程度的代谢，其中血清代谢较迅速，其主要代谢产物为相应单酯类化合物，包括邻苯二甲酸单乙酯（mEP）、邻苯二甲酸单丁酯（mBP）、邻苯二甲酸单苄酯（mBzP）及邻苯二甲酸．单．乙基己基酯（mEHP）。由此说明邻苯二甲酸酯类在草鱼脏器中主要被组织水解酶所水解，血液和小肠是此类化合物经鳃和口染毒的第一代谢转化场所。

英文摘要：

In order to reveal the toxicity mechanism of PAEs chemicals, the article developed a method for simultaneous determination of diethyl phthalate （DEP）, dibutyl phthalate （DBP）, butyl benzyl phthalate （BBP） and di-（2-ethylhexyl） phthalate （DEHP） by using GC-FID. Meanwhile, the metabolism of four PAEs chemicals was also studied in Ctenopharyngodon idellus organs and hepatocyte through LC-MS/MS. The results showed that PAEs chemicals were not metabolized significantly in hepatocyte, but they were metabolized quickly in blood serum, intestinal tract and hepato-pancreas homogenate, especially in the serum. And the main metabolites were mono-phthalates, including monoethyl phthalate （mEP）, monobutyl phthalate （mBP）, monobenzyl phthalate （mBzP） and monoethylhexyl phthalate （mEHP）. Therefore, it was concluded that the PAEs chemicals were mainly hydrolyzed by means of the proteolytic enzymes in C. idellus organs, and the blood and intestine were the first metabolized location of PAEs chemicals absorbed by the fish gill and intestine.