中文摘要：

为研究鸡传染性贫血病毒（CIAV）VP3蛋白在宿主细胞中的作用,本研究采用PCR技术从CIAV DNA中扩增出vp3基因,并将该基因克隆到p MD18-T simple vector中。经测序证实所克隆的vp3基因与CIAV CUX-1标准株相同。并将vp3基因亚克隆到真核表达载体p EGFP-N1中,构建了重组真核表达质粒p EGFP-vp3与p CMV-Myc vp3,转染BHK-21和293 T细胞,经RT-PCR、荧光显微镜以及Western Blot证实转染的真核细胞能够表达VP3蛋白。以台盼蓝染色法检测细胞死亡,结果表明,VP3蛋白具有较强诱导细胞死亡的作用。该结果为深入研究CIAV的致病机理奠定了基础。

英文摘要：

In order to elucidate the role of chicken infectious anemia virus（CIAV）VP3 protein in host cells,vp3 gene was cloned from the viral DNA by PCR and was inserted into the p MD18-T simple vector. The result of DNA sequencing showed that the sequence of vp3 gene was the same as that of CUX-1.The vp3 gene was then sub-cloned into the eukaryotic expression vector p EGFP-N1 or p CMV-Myc.The p EGFP-vp3 or p CMV-Myc-vp3 plasmids were transfected into the BHK-21 or 293 T cells for the expression of VP3.The expression of VP3 by the transfected cells was determined by RT-PCR,fluorescence microscope and Western Blot.VP3-induced cell death was measured by the Trypan Blue-staining.The results showed that the protein VP3 could be expressed in the transfected cells and induce cell death.These data lay a foundation for elucidation of pathogenesis of CIAV.