中文摘要：

为了深入研究禽呼肠孤病毒（ARV）的致病机理,从其基因组中克隆病毒非结构蛋白p10基因,并将其与原核表达载体连接,转入大肠杆菌BL21中,使其表达携带His标签的p10融合蛋白,经镍柱纯化得到高纯度重组p10蛋白,以该蛋白为免疫原免疫BALB/c小鼠,将免疫小鼠的脾细胞与骨髓瘤细胞（SP2/0）进行融合,经过3次亚克隆后获得2株能够稳定分泌针对p10蛋白的杂交瘤细胞株,分别命名为2B12与4H10.通过间接ELISA（Enzyme-linked immunosorbentassay）方法测定其腹水效价均为1∶1.01x10^7,亲合力解离常数（kD）分别为3.91x10-10、5.75x10-10,2株单抗的IgG亚型均为IgG2b.经Western Blot鉴定,该2株抗体均能特异性识别ARV感染DF-1细胞中的p10蛋白.这些单抗的制备为深入研究ARV的致病机理奠定了基础.

英文摘要：

In order to investigate the pathogenesis of avian reov：rus（ ARV ）, we cloned p10 gene from ARV into prokaryotic ex-pression vector pET28a. The expression vector was transformed into E.coli BL21 for the expression of p10. Fusion protein p10-Hiswas purified with a Ni-NTA affinity column and was used as an immunogen to vaccinate BALB/C mice. Hybridomas were devel-oped by fusing mouse myeloma ceils SP2/0 and splenocytes from the immunized mice and screened with purified p10 for the posi-tive clones. Two hybridomas secreting antibodies against p10 were obtained and named 2B12 and 4H10, respectively. Western Blot-analysis showed that both of them could specifically bind to pI0 in ARV-infected cells.The titers of ascites from the hybridoma-in-jected mice were both 1 ： 1.01x 10^7as determined by an indirect ELISA test. The isotypes of the monoelonal antibodies were deter-mined to be IgG2b. The dissociation constants （kD）of these two monoclonal antibodies were determined to be 3.91x 10-1 and 5.75x10-1 respectively. These antibodies will be of great assistance to elucidate the pathogenesis of Avian Reovirus.