在前期研究中发现,鸡卵清提取液有促细胞存活与增殖的作用。在该研究中,作者进一步分离鸡卵清提取液的各成分,找到促细胞增殖的主要成分。用超滤管将鸡卵清提取液分为大于3 kDa和小于3 kDa的成分,用于细胞增殖活性测定,发现鸡卵清提取液中小于3 kDa的成分有促293T细胞增殖的作用。可将鸡卵清提取液分离小于3 kDa的成分作为促293T细胞增殖的有效成分。
We found that chicken egg extract could promote cell survival and proliferation. In this study, we further separated the components of chicken egg extract to find the main ingredient promoting cell proliferation. The chicken ovalbumin extract was separated into more than 3 kDa and less than 3 kDa components by ultrafiltration, and then their effect on cell proliferation activity were determined. Less than 3 kDa ingredients in chicken ovalbumin extract can promote 293 T cell proliferation. We can separate less than 3 kDa fraction in the chicken ovalbumin as an active ingredient to promote 293 T cell proliferation.