中文摘要：

背景：系统性红斑狼疮是一种以多器官或多系统病变和血清中出现多种自身抗体为特征的自身免疫性疾病，目前缺乏有效的治疗方案，而理论上间充质干细胞可用于治疗系统性红斑狼疮。目的：观察人脐带间充质干细胞移植治疗系统性红斑狼疮小鼠的疗效。方法：分离培养人脐带间充质干细胞，并用深红色荧光DiR标记细胞。实验小鼠分5组：正常对照组（C57BL小鼠），模型对照组（C57BL/lpr小鼠），低、中、高剂量脐带间充质干细胞治疗组（C57BL/lpr小鼠），每组10只。各治疗组通过尾静脉注射低、中、高剂量（2×106，1×106，0.5×106结果与结论：细胞移植3次后，外周血抗核抗体、抗组蛋白抗体、抗双链DNA抗体均明显下降，CD4个）脐带间充质干细胞，每周1次，连续3周，治疗结束采血测抗核抗体、抗组蛋白抗体、抗双链DNA抗体变化，定量PCR检测OPG和Foxp3基因表达的变化。＋CD25＋T细胞明显升高，OPG和Foxp3基因表达也明显升高，接近正常对照组，与模型对照组相比差异均有显著性意义（P 〈0.01）。结果表明人脐带间充质干细胞能使C57BL/lpr小鼠的各项相关指标恢复到C57BL正常鼠水平，以高剂量治疗组效果最明显。

英文摘要：

BACKGROUND：Systemic lupus erythematosus is an autoimmune disease characterized as an emergence of a variety of autoantibodies in serum and multi-system and multi-organ lesions. Currently, there is a lack of effective treatment options, and umbilical cord mesenchymal stem cel s are a promising therapy for systemic lupus erythematosus based on cel biological roles. OBJECTIVE：To observe the therapeutic efficacy of human umbilical cord mesenchymal stem cel transplantation in the treatment of systemic lupus erythematosus in mice. METHODS：Human umbilical cord mesenchymal stem cel s were isolated and cultured fol owed by labeling with DiR fluorescence. Experimental mice were divided into normal control group （C57BL mice）, model control group （C57BL/lpr mice）, low-, medium-and high-dose umbilical cord mesenchymal stem cel therapy groups （C57BL/lpr mice）, with 10 mice in each group. Mice in the low-, medium-and high-dose groups were respectively injected 0.5×106, 1×106, 2×106 human umbilical cord mesenchymal stem cel s, once a week, for 3 consecutive weeks. At the end of treatment, blood samples were col ected to measure antinuclear antibody, anti-histone antibody, anti-double stranded DNA antibody changes;OPG and Foxp3 gene expression changes were detected by quantitative PCR method. RESULTS AND CONCLUSION：After treatment, the levels of anti-nuclear antibodies, anti-histone antibodies and anti-double stranded DNA antibodies in the peripheral blood of mice were al declined in the low-, medium-and high-dose groups, while the number of peripheral blood CD4＋CD25＋T cel s was significantly elevated. OPG and Foxp3 gene expression was also increased dramatical y in the low-, medium-and high-dose groups, which was similar to that in the normal control group and significantly different from that in the model control group （P〈0.01）. Experimental findings demonstrate that after transplantation of human umbilical cord mesenchymal stem cel s, al relevant indicators in C57BL/lpr mice recovered to the no