中文摘要：

探讨1，25（OH）2D3对2型糖尿病（T2DM）患者单核细胞的免疫调节作用。体外培养的外周单核细胞分别从23例正常对照和22例T2DM患者中获得。观察1，25（OH）：D3预干预后，单核细胞对TLR4配体（脂多糖，LPS）的反应性变化以及单核细胞表面CD14和TLR4表达变化。流式细胞仪分析单核细胞CD14和TLR4表达以及NF—KB—p65磷酸化水平，IL-1β和TNF-α水平用酶联免疫吸附法（ELISA）检测。T2DM单核细胞表面TLR4表达高于正常对照组（P〈0．05）；LPS使正常对照组单核细胞表面TLR4表达下降的幅度明显高于T2DM组（P〈0．05）。LPS干预后T2DM单核细胞的NF—KB—p65磷酸化水平和IL-1β和TNF-α浓度均明显高于正常对照组（P〈0．001）；1，25（OH）2D3预干预后，LPS对单核细胞表面CD14和TLR4表达、NF—KB—p65磷酸化水平和IL-1β、TNF-α浓度的影响在T2DM和对照组间比较无差异（P〉0．05）。研究结果表明1，25（OH）2D3对T2DM患者单核细胞的高反应性具有一定的免疫调节作用，这有助于我们近一步地认识和探讨1，25（OH）2D3对T2DM的固有免疫调节的作用。

英文摘要：

To investigate modulation of 1,25 -dihydroxy -vitamin D3 on monocyte activity from T2DM. Peripheral blood monocytes were respectively collected from 23 healthy controls and 22 type 2 diabetes mellitus （T2DM） ,respectively. The effect of 1,25 - dihydroxy - vitamin D3 （ 1,25 （ OH ） 2 D3 ） on monocyte response to TLR4 ligand （LPS） was evaluated in vitro by measuring phosphorylation level of NF - KB - p65 and associated cytokine production （IL-1β and TNF-α）. In additional,effects of preincubation with 1,25 （OH）2D3 on moncyte CD14 and TLR4 expression stimulated by LPS were observed. CD14 and TLR4 expression and phosphorylation level of NF - KB - p65 were determined by flow cytometer （FCM）. IL-1β and TNF-α concentrations were measured by en- zyme- linked immunosorbent assay （ELISA）. Monocytes from T2DM showed higher TLR4 expression than that from controls （ P 〈 0.05 ）. After incubation with LPS, the levels of monocyte TLR4 expression remarkably decreased in controls, as compared with T2DM （ P 〈 0.05 ）. Additionally, expressions of CD14 and TLR4 on monocytes surface did not show significant changes between T2DM and controls （P 〉 0.05 ） ,when monocytes were pretreated with 1, 25 （OH） 2 D3 and afterwards incubated with LPS. Activation of NF - KB and amounts of IL-1β and TNF-α production by stimulation with LPS significantly increased in T2DM, which was modulated by 1,25 （OH）2 D3 to similar level,as compared to controls. Monocytes from T2DM showed higher cellular reactivity towards LPS and 1,25 （OH） 2 D3 was observed to restore this defect to a certain extent in vitro. The modulation of 1,25 （OH） 2 D3 on monocytes makes us to consider more potency of immunoregulation by vitamin D3 in T2DM.