中文摘要：

瑞斯强风，由真菌的病原体 Magnaporthe oryzae 引起了，是世界范围的大多数破坏庄稼疾病之一。相应于在地 isolate CHL346 的大米强风抵抗基因 Pi7 的无毒性基因作为单个基因被继承，指明的 AvrPi7，在由子孙从在地之间的一个十字导出的 189 ascospore 组成的一张分离人口孤立 CHL346 和 CHL42。以便决定 AvrPi7 地点的 chromosomal 地点， 121 简单顺序重复(SSR ) 的一个总数标记基于 M 的参考 isolate 70-15 的整个染色体的顺序被开发。oryzae。有这些 SSR 标记的地点的连接分析证明染色体 1 上的八个 SSR 标记被连接到地点，最靠近的 flanking 标记 MS1-9 和 MS1-15 分别地在之中是从地点的 3.2 和 16.4 厘米。为好印射，包括八个 SSR 标记和三个候选人无毒性基因(CAG ) 标记的另外的基于 PCR 的制造者两个都在区域 flanking 被开发标记。AvrPi7 地点被标记 MS1-21 和 MS1-22 遗传上在 1.6 厘米的区域 flanked 以内限定，并且与标记 CAG2 共同分离。构造 AvrPi7 地点的一张物理地图，连接到 Avrgene 的分子的标记通过简历信息分析(BIA ) 在参考 isolate 70-15 的 supercontigs 上被印射。因而， AvrPi7 地点被标记 MS1-21and MS1-22 基于参考顺序限定到 75-kb 间隔 flanked。Merodiploids 在这观察到学习也被讨论。

英文摘要：

Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating crop diseases worldwide. The avirulence gene corresponding to rice blast resistance gene Pi7 in field isolate CHL346 was inherited as a single gene, designated AvrPiT, in a segregating population consisting of 189 ascospore progenies derived from a cross between field isolates CHL346 and CHL42. In order to determine the chromosomal location of the AvrPi7 locus, a total of 121 simple sequence repeat （SSR） markers were developed based on the whole-genome sequence of reference isolate 70-15 of M. oryzae. Linkage analysis of the locus with these SSR markers showed that eight SSR markers on chromosome 1 were linked to the locus, among which the closest flanking markers MS1-9 and MS1-15 were 3.2 and 16.4 cM from the locus, respectively. For fine mapping, additional PCR-based makers including eight SSR markers and three candidate avirulence gene （CAG） markers were developed in the region flanking both markers. The AvrPi7 locus was genetically delimited within a 1.6-cM region flanked by markers MS1-21 and MS1-22, and co-segregated with the marker CAG2. To construct a physical map of the AvrPi7 locus, molecular markers linked to the Avr gene were mapped on the supercontigs of the reference isolate 70-15 through bioinformation analysis （BIA）. Consequently, the AvrPi7 locus was delimited to a 75-kb interval flanked by markers MS1-21 and MS1-22 based on the reference sequence. Merodiploids observed in this study are also discussed.