中文摘要：

UDP 葡萄糖 pyrophosphorylase (UGPase ) 是主要在场的并且在植物在糖类新陈代谢起重要作用。二相应 UGPase 基因， OsUgp1 和 OsUgp2，在米饭存在染色体。OsUgp1 最近被报导了象为种子糖类新陈代谢一样在花粉母亲房间和成熟分裂阶段期间为愈伤葡聚糖免职必要。在这研究， OsUgp2 的全身的 cDNA 从米饭花药被孤立。在 situ 杂交的北污点和 RNA 显示 OsUgp2 的表示是优先地在花粉并且精神上发展调整。没有抄本在单核的小孢子前在叶，茎， lemma/palea，成熟谷物和小花被发现发展舞台，而是 OsUgp2 mRNA 的大数量在双性人在花粉被发现起核心作用并且成熟阶段。OsUgp2 的免疫本地化在 situ 杂交由 RNA 显示出一个类似的表示模式到那。OsUgp2 的功能被调停 dsRNA 的 transcriptional 基因 silencing 调查。16 独立转基因的米饭植物的花粉富饶在 25% 和 90% 之间被发现，它与 OsUgp2 mRNA 的数量被相关。词法变化和淀粉变化的结果在在转基因的米饭的花粉开发期间证明花粉开发的反常特征出现在单核的小孢子阶段以后。淀粉没能在花粉积累并且因此导致了无菌的花粉。这些结果证明 OsUgp2 是“花粉优先的迟了的基因”并且在花粉成熟期间起一个关键作用，特别为淀粉累积。OsUgp2 补充 OsUgp1 完成 UGPase 为花粉开发的完整的进程必要的函数。

英文摘要：

UDP-glucose pyrophosphorylase （UGPase） is predominantly present and plays significant role in carbohydrate metabolism in plants. Two homologous UGPase genes, OsUgp1 and OsUgp2, exist in rice genome. OsUgpl has recently been reported to be essential for callose deposition during pollen mother cell and meiosis stages as well as for seed carbohydrate metabolism. In this study, a full-length cDNA of OsUgp2 was isolated from rice anther. Northern blot and RNA in situ hybridization indicated that the expression of OsUgp2 was preferentially in pollen and developmentally regulated. No transcripts were found in leaf, stem, lemma/palea, ripening grain and florets before the uninucleate microspore developmental stage, but a large quantity of OsUgp2 mRNA was found in pollen at the binucleate and mature stages. The immunolocalization of OsUgp2 showed a similar expression pattern to that by RNA in situ hybridization. The function of OsUgp2was investigated by dsRNA-mediated transcriptional gene silencing. The pollen fertility of 16 independent transgenic rice plants was found between 25% and 90%, which was correlated with the amount of OsUgp2 mRNA. The results of morphological changes and starch variation during pollen development in transgenic rice showed that the abnormal feature of pollen development appeared after the uninucleate microspore stage. Starch failed to accumulate in pollen and thus led to sterile pollens. These results demonstrated that OsUgp2 is a pollen-preferential ＂late gene＂ and plays a key role during pollen maturation, especially for starch accumulation. OsUgp2 complements OsUgpl to fulfill the UGPase＇s functions necessary for＇the full process of pollen development.