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Construction of an electronic physical map of Magnaporthe oryzae using genomic position-ready SSR markers
  • ISSN号:1007-7146
  • 期刊名称:《激光生物学报》
  • 时间:0
  • 分类:R284[医药卫生—中药学;医药卫生—中医学]
  • 作者机构:[1]Laboratory of Plant Resistance and Genetics, College of Natural Resources and Environmental Science, South China Agricultural University, Guangzhou 510642, China, [2]Laboratory of Plant Protection, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
  • 相关基金:Supported by the National Basic Research Program of China (Grant No. 2006CB100206), Program for Changjiang Scholars and Innovation Research Team in University (Grant No. IRT0448) and the Natural Science Foundation of Guangdong Province (Grant No. 039254, 07300695)
中文摘要:

Magnaporthe oryzae 是为植物的一个模型病原的细丝状的真菌。我们集合了一次简单顺序重复(SSR ) 基于种类的物理地图,用在 silico 顺序数据。一套 120 个 SSR 标记从参考 isolate 70-15 的 genomic 顺序被开发。这些标记乐意地从其它的直接存储器存取孤立的 genomic 被放大,并且突变而产生之遗传的变化的高水平描述了父母测试的二个十字孤立。所有标记是到七 M 之一的基因座桌子。oryzae 染色体。一基于 SSR 在 silico 物理地图被构造,并且先存在 SSR 和 RFLP loci 集成于地图,与 23 4w (无毒性) 一起基因和到植物 / 病原体相互作用的重要性的二另外的基因。Thismap 为人口遗传提供一个平台,功能的基因组学在模型病原体,并且甚至在另外的 evolu-tionally 相关的病原体学习。

英文摘要:

Magnaporthe oryzae is a model for plant pathogenic filamentous fungi. We have assembled a simple sequence repeat (SSR)-based physical map of the species, using in silico sequence data. A set of 120 SSR markers was developed from the genomic sequence of the reference isolate 70-15. These markers were readily amplified from the genomic DNA of other isolates, and high levels of allelic variation characterised the parental isolates of the two crosses tested. All the markers were Iocatable to one of the seven M. oryzae chromosomes. An SSR-based physical in silico map was constructed, and pre-existing SSR and RFLP loci were integrated into the map, along with 23 Avr(avirulence) genes and two other genes of importance to the plant/pathogen interaction. This map provides a platform for population genetics and functional genomics studies in the model pathogen, and even in other evolu- tionally related pathogens.

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期刊信息
  • 《激光生物学报》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国遗传学会
  • 主编:胡能书
  • 地址:长沙市麓山路36号湖南师范大学生命科学学院内
  • 邮编:410081
  • 邮箱:jgswxb@hunnu.edu.cn
  • 电话:0731-8872208
  • 国际标准刊号:ISSN:1007-7146
  • 国内统一刊号:ISSN:43-1264/Q
  • 邮发代号:42-194
  • 获奖情况:
  • 2000年12月荣获首届《CAI-CD》执行优秀奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊
  • 被引量:5516