中文摘要：

目的探讨抗Jo-1抗体水平与多发性肌炎，皮肌炎疾病活动度的相关性。方法采用免疫印迹法和酶联免疫吸附试验（ELISA）法检测148例多发性肌炎，皮肌炎患者和性别年龄相匹配的130名健康人血清抗Jo-1抗体水平，利用国际肌炎组织制定的炎性肌病评价工具评估抗Jo-1抗体阳性患者疾病活动度（VAS），并分析抗Jo-1抗体水平与多发性肌炎／皮肌炎疾病活动度的相关性。统计学处理采用x2检验或t检验。结果免疫印迹法和ELISA法检测多发性肌炎，皮肌炎患者血清抗Jo-1抗体的阳性率分别是24．3％（36／148）和27．0％（40／148），健康人血清中抗Jo-1抗体均为阴性。发热、肺间质病变（ILD）和关节炎，关节痛在抗Jo-1抗体阳性组的比例明显高于抗Jo-1抗体的阴性组（P〈0．05）。抗Jo-1抗体阳性患者的总疾病活动度评分与血清抗Jo-1抗体的浓度呈正相关（，==0．874，P=0．000）。7例患者治疗后患者血清抗Jo-1抗体浓度与疾病活动度评分均显著下降，两者变化一致。结论ELISA法检测抗Jo-1抗体水平与疾病活动相关，可作为多发性肌炎，皮肌炎疾病活动的评价指标。

英文摘要：

Objective To evaluate the correlation between serum anti-Jo-1 antibody level and myositis disease activity in polymyositis/dermatomyositis. Methods Anti-Jo-1 antibody levels in serum from 148 polymyositis/dermatomyositis patients and 130 healthy controls were measured by both ELISA and immunoblot assay. Disease activity of the anti-Jo-1 antibody positive patients was assessed by the global myositis disease activity score （visual analogue score, VAS） established by the International Myositis Assess- ment and Clinical Studies （IMACS） Group. The correlation between disease activity and level of serum anti- Jo-1 antibody was assessed. Comparisons between groups were performed by X2 test or t test. Results The positive rate of ELISA and EUROLINE was 24.3%（36/148） and 27.0%（40/148） respectively. Fever, ILD, arthritis/arthralgia were found to be more predominant in anti-Jo-1 antibody positive patients than those who were anti-Jo-1 antibody negative patients. There was significant positive correlation between the global myositis disease activity score （VAS） and serum level of anti-Jo-1 antibody （r=-0.874, P=-9.000）. Serum anti- Jo-1 antibody levels, together with global disease activity, were significantly decreased in the 7 patients after treatment. Conclusion Anti-Jo-1 antibody level measured by ELISA is associated with disease activity of PM/DM, and could be a marker of disease activity.