中文摘要：

目的 通过观察自身免疫性炎性肌病（EAM）大鼠肌肉和肺组织Ⅰ型IFN系统的表达，初步探讨EAM合并肺间质病变的病理机制。方法 建立EAM大鼠模型，分为健康对照组、EAM模型对照组、EAM模型组，测3组大鼠血清中肌酸激酶（CK）水平，HE染色分析模型大鼠肌肉和肺脏病变程度，实时定量PCR测Ⅰ型IFN系统在肌肉和肺组织中的表达。结果 与健康对照组、EAM模型对照组比，EAM模型组血清CK[（209.17±91.95） IU/L]、肌肉病理评分[（2.17±0.76）分]和肺脏组织病理评分[（1.75 ±0.41）分]、肌肉组织中Ⅰ型IFN系统mRNA表达水平明显升高，差异有统计学意义（P值均小于0.05）。与EAM模型对照组比，EAM模型组肺组织IFNα、IFNβ、干扰素α受体1（IFNαR1）、信号传导蛋白和转录激活物1（STAT1）、黏病毒抑制蛋白1（MX1） mRNA表达水平升高，差异有统计学意义（P值均小于0.05），而干扰素诱导蛋白1（IFIT1）、干扰素诱导基因15（ISG15）表达水平差异无统计学意义。EAM模型组大鼠肌肉组织中IFNα、IFNβ、IFNαR1、STAT1、MX1、IFIT1、ISG15 mRNA表达与血清CK水平和肌肉病理评分呈正相关。EAM模型组大鼠肺组织中IFNα、IFNβ、IFNαR1、STAT1、MX1 mRNA表达与肺脏病理评分呈正相关。结论 EAM大鼠肌肉和肺组织均有Ⅰ型IFN系统表达，Ⅰ型IFN可能参与了EAM大鼠靶器官的病理损害。

英文摘要：

Objective To investigate the expression levels of the type Ⅰ IFN system in muscle and lung of experimental autoimmune myositis （EAM） model and to evaluate whether the type Ⅰ IFN system associates with the pathogenesis of the EAM model in rats.Methods The EAM model was established to determine creatine kinase （CK） in blood serum.The pathology of muscle and lung tissue was examined by hematoxylin-eosin staining.The concentration of type Ⅰ IFN system mRNA in muscle and lung tissue was detected by real-time PCR.Results The concentration of CK in model group [（209.17 ±91.95） IU/L]was significantly higher than that of two control groups（P 〈0.05）.The scores of muscle and lung in EAM model were significantly higher than that of control groups （all P 〈 0.05）.The expression levels of the type Ⅰ IFN system in muscle of EAM model were significantly higher than that of control groups（all P 〈0.05）.The expression levels of the type Ⅰ IFN system in muscle with EAM model were positively correlated with CK and the scores of muscle （all P 〈 0.05）.The expression levels of IFNα, IFNβ, IFNαR1, signal transducer and activator of transcription 1 （STAT1）, myxovirus resistance protein 1 （MX1） in lung of EAM model were significantly higher than those of control groups（P 〈 0.05）, but not seen in INF-induced protein with tetratricopetide repeats 1 （IFIT1） and IFN-stimulated gene 15 （ISG15）.The expression levels of IFNα, IFNβ, IFNαR1, STAT1 and MX1 in lung with EAM model were positively correlated with the scores of lung pathology （all P 〈 0.05）.Conclusion The type Ⅰ IFN system probably played a crucial role in the pathogenesis and the pathology of muscle and lung of EAM model.