中文摘要：

[摘要]目的探讨N-乙酰-丝氨酰-天门冬酰-赖氨酰-脯氨酸（AcSDKP）对小鼠RAW264．7巨噬细胞促炎功能的影响。方法取小鼠RAW264．7巨噬细胞体外培养，分为对照组、脂多糖（Lps）组和LPS加不同浓度的AcSDKP（1nmol／L、10nmol／L和100nmol／L）处理组。采用荧光定量PCR法检测细胞肿瘤坏死因子（TNF）-α、白细胞素（IL）-1β、IL-6和诱导型-氧化氮合成酶（iN0S）mRNA相对水平；使用Transwell小室检测RAW264．7细胞的趋化能力；采用Westernblot法检测细胞iN0S、磷酸化的蛋白激酶复合体抑制物（p—IKK）、磷酸化的KB抑制蛋白（p—IKB）和磷酸化的P65（p—P65）蛋白表达水平。结果LPS处理组TNF—α、IL-1β和IL-6mRNA水平显著高于对照组[分别高出（41±2．1）倍、（1073±80．8）倍和（1726±110．2）倍，P〈0．01]；AcSDKP（10nmol／L和100nmol／L）处理组TNF-α水平显著低于LPS处理组[分别降低19．0％和39．3％，P〈0．01]；AcSDKP（1nmol／L、10nmol／L和100nmol／L）处理组IL-1口水平显著低于LPS处理组[分别降低22．08％、35．8％和38．2％，P〈0．01]；AcSDKP（1nmol／L、10nmol／L和100nmol／L）处理组IL-6水平显著低于LPS处理组[分别降低15．8％、35．7％和43．3％，P〈0．01]；LPS处理组穿过小室的细胞数为（136±5-3）个／HP，显著高于对照组[（64±5．3）个／HP，P〈0．01]，而AcSDKP处理组穿过小室的细胞数[分别为（105±4．8）、（85．3±5．0）和（73．7±5．6）个／HP]，显著低于LPS组[（136±5．3）个／HP，P〈0．05]；LPS处理组iN0SmRNA和蛋白表达水平显著高于对照组[分别为（21±2．5）倍和（5．9±0．4）倍，P〈0．01]，而AcSDKP处理组iN0SmRNA显著低于LPS处理组[分别降低37．8％、23.3％和33．1％，P〈0．05]；在10nmot／L和100nmol／L浓度AcSDKP处理组，两种蛋白水平显著低于LPS处理组[分别降低27．0％和40．2％，P〈0．05]；LPS处理组p-IK

英文摘要：

Objective To investigate the effect of N-acetyl-seryl-aspartyl-lysyl-protine （AcSDKP） on mouse RAW264.7 macrophage cell line. Methods RAW264.7 mouse macrophage cells were cultured in vitro. Cells were divided into five groups, namely the control group （vehicle only）, lipopolysaccharides （LPS） group （LPS only）, and AcSDKP intervention groups （LPS plus AcSDKP at 1 nmol/L, 10 nmol/L and 100 nmol/L）. The mRNA levels of tumor necrosis factor-α （TNF-α）,interleukin （IL）-Iβ,IL-6 and inducible nitric oxide synthase （iNOS） were measured by real-time RT-PCR. The chemotactic motility of RAW264.7 cells was detected by using Transwell assay. Western blot was performed to determine the iNOS,phosphorylated inhibitor of KB protein kinase complex （p-IKK）,phosphorylated inhibitor of KB （p-IKB） and phosphorylated P65 （p-P65） expression. Results The mRNA levels of TNF-ct,IL-I[3 and IL-6 in LPS group were increased by （41±2.1）, （1073±80.8） and （1726±110.2） folds eompared with in control group, respectively （P〈0.01）;the TNF-a mRNA levels in AeSDKP （1 nmol/L,10 nmol/L and 100 nmol/L） treatment groups were decreased by 8.1% （P〉0.05）, 19.0% （P〈0.01） and 39.3% （P〈0.01）,respectively as compared with in LPS group , IL -1β were decreased by 22.0%,35.8% and 38.2%,respectively, （P〈0.01）,and IL-6 were decreased by 15.8%,35.7% and 43.3%, respectively （P〈0.01）;The migrated cell counts across the chamber membrane in LPS group （136±5.3）/HP were significantly higher than those in control group [（64±5.3）,P〈0.01],however,the cell counts in AeSDKP treatment groups [（105±4.8） /HP, （85.3±5.0）/HP and （73.7±5.6）/HP,P〈0.05] were significantly lesser than those in LPS group （P〈0.05）;The mRNA and protein levels of iNOS were increased by （21±2.5） and （5.9±0.4） folds compared with those in the eontrol group,However,the mRNA levels of iNOS in AeSDKP treatment groups were decreased by 37.8%,23.3% and 33.1%,respectively