中文摘要：

摘要从中国典型培养物保藏中心（CCTCC）引进HighFive细胞并对其进行驯化，获得无血清悬浮驯化株（Hi5-SF）。依据《中华人民共和国药典（2010年版三部）》对其进行细胞生物学特性研究、微生物污染、病毒外源因子及细胞致瘤性检测。结果发现，Hi5-SF细胞复苏活力76．3％，生长曲线呈“s”型，最大增殖密度可达3．50×10^6mL^-1，群体倍增时间为26．2h；细菌、真菌及支原体检查均为阴性；血细胞吸附试验、致细胞病变试验和特异性病毒荧光抗体结合物检查结果均为阴性；致瘤性检查结果也为阴性。说明该细胞株符合兽用疫苗生产用细胞质量要求，可以在疫苗研究与生产中应用。

英文摘要：

High Five cells were introduced from CCTCC and domestecated, ,and we obtained semi-ad- herent cells to suspension growth cell line （Hi5-SF）. Subsequently, the biological characteristics, mi- crobial contamination,viral exogenous factors and tumorigenicity of Hi5-SF cell line were detected ac- cording to the Pharmacopoeia of People＇s Republic of China （3rd Edition,2010）. The results showed that the recovery vitality of Hi5-SF cells was 76.3%, the growth curve was S-shape, and maximum concentration was 3.50×10^6 cells ·mL^-1 with the population doubling time（PDT） of 26. 2 h. The cells were free of bacteria, fungi and mycoplasmas~ hemadsorption test, cytopathic test, specific virus fluorescent antibody binding test and tumorigenicity test were all negative. The resuhus indicated that the HiS-SF cell line meets the quality requirements of veterinary vaccine production.