中文摘要：

目的：建立脑心肌炎病毒（EMCV）免疫球蛋白 M （IgM ）捕获酶联免疫吸附试验（ELISA）检测方法，用于感染 EMCV 血清学早期诊断。方法用抗人 IgM （μ链）单抗进行包被，辣根过氧化物酶（HRP）标记的 EMCV为酶标抗原，初步建立 EMCV IgM 捕获 ELISA ；对建立的方法进行条件优化及特异性、精密性、稳定性等验证。结果建立的 EMCV IgM 捕获 ELISA 检测方法抗体包被浓度为1μg／mL ，EMCV‐HRP 工作浓度和反应时间分别为1∶400和45 min ，封闭液为10％马血清时该法检测效果可达最佳，3批试剂批内及批间变异系数均小于5％，且特异性、精密性及稳定性较好。结论建立的 EMCV IgM 捕获 ELISA 法特异、精密且稳定，可用于人感染 EMCV的早期检查，为 EMCV 的诊断奠定基础。

英文摘要：

Objective To establish the encephalomyocarditis virus（EMCV） immunoglobulin M （IgM ） captured enzyme‐linked immunosorbent assay （ELISA ） detection method for the application in early serological diagnosis of EMCV infection .Methods Anti‐human（μ chain） monoclonal antibody was used to conduct the coating and the horse radish peroxidase（HRP） labelled EMCV served as the enzyme labeled antigen ,the EMCV IgM capture ELISA meth‐od was preliminarily established ;The established method was performed the conditional optimization and verification of the specificity ,precision and stability .Results The antibody coating concentration in the established EMCV IgM captured ELISA method was 1 μg/mL ,the EMCV‐HRP work concentration and reaction times were 1 ∶ 400 and 45 min respectively .When the blocking solution was 10% horse serum ,this method achieved the best effect .The intra‐assay and inter‐assay coefficients of variation in 3 batches of reagents were less than 5% ,and the specificity ,precision and stability were better .Conclusion The established EMCV IgM capture ELISA method is specific ,precise and sta‐ble ,can be used for the detection of early human EMCV infection and lays the foundation for the EMCV diagnosis .