中文摘要：

这封信报导一个 chemiluminescene 免疫分析方法与 immunomagnetic 分离结合了很快检测 Cyfra 21-1，在 bio-functionlized，磁性的 nanocomposites 作为活动底层被使用捕获并且孤立 cyfra 21-1 蛋白质。在捕获的 Cyfra 以后， 21-1 进一步与山葵反应了形成三明治 immunocomplex 的结合 peroxidase 的 anti-Cyfra 21-1 抗体，化合光将由于 chemiluminescent 底层的增加被生产。一个自制光生物传感器被设计检测化合光而不是另外的大仪器。在化合光紧张和在从 0.2 ～ 50 ng/mL 的范围的 Cyfra 21-1 的集中之间有好线性回答。包括孵化的整个察觉过程，洗并且察觉能在 45 min 以内被执行。建议方法提议一简单，为检测非小的房间肺癌症的 noninvasive 和可靠工具并且为临床的测试有潜在的申请。

英文摘要：

This letter reports a chemiluminescene immunoassay method combined with immunomagnetic separation to rapidly detect Cyfra 21-1, in which bio-functionlized magnetic nanocomposites were used as mobile substrate for capturing and isolating the cyfra 21-1 proteins. After the captured Cyfra 21-1 further reacted with horseradish peroxidase-conjugated anti-Cyfra 21-1 antibody to form a sandwich immunocomplex, the chemiluminescence would be produced as a result of addition of the chemiluminescent substrate. A home-made optical biosensor was designed to detect the chemiluminescence instead of other large instruments. There is a good linear response between the chemiluminescence intensity and the concentration of Cyfra 2 I-1 in the range from 0.2 to 50 ng/mL. The whole detection process including incubation, washing and detection could be performed within 45 min. The pro- posed method offers a simple, noninvasive and reliable tool for detecting non-small cell lung cancer and has potential application for clinical testing.