中文摘要：

目的检测淋巴管内皮细胞透明质酸受体1（LYVE-1）和同源盒基因转录因子1（PROX-1）在人乳腺癌组织中的表达,探讨淋巴管密度在肿瘤淋巴结转移中的临床意义。方法收集80例乳腺浸润性导管癌和35例乳腺良性增生组织,采用免疫组织化学SP法检测LYVE-1和PROX-1的表达。结果乳腺癌组织中LYVE-1和PROX-1各自标记的淋巴管密度均明显高于乳腺增生组织,差异均有统计学意义（均P〈0.01）,且与肿瘤淋巴结转移密切相关,有淋巴结转移者均高于无淋巴结转移者（均P〈0.01）;PROX-1在癌细胞内的表达,不同的肿瘤病理学分级和临床分期之间差异均有统计学意义（均P〈0.01）。结论乳腺浸润性导管癌组织中淋巴管生成增多,淋巴管密度与肿瘤淋巴结转移相关,PROX-1可能与乳腺癌发生、发展及淋巴结转移相关。

英文摘要：

Objective To investigate the expressions of LYVE-1and PROX-1in human breast carcinoma and the clinical significance of lymphatic vessel density.Methods Immunohistochemistry（SP method）was used to detect the expressions of LYVE-1and PROX-1in 80 specimens of breast invasive ductal carcinoma and 35 specimens of breast hyperplasia.Results The density of lymphatic vessels positive for LYVE-1or PROX-1was significantly higher in breast carcinoma than in breast hyperplasia（P〈0.01）.There was a significant correlation between lymphatic vessel density and lymph node metastasis（P〈0.01）.A negative correlation was noted between the PROX-1expression in carcinoma cells and tumor grade（P〈0.01）or TNM stage（P〈0.01）.Conclusion Lymphangiogenesis is increased in breast carcinoma,which is associated with lymph node metastasis.PROX-1may be involved in tumorigenesis,progression and lymphatic metastasis in breast cancer.