中文摘要：

用海藻酸钠-淀粉凝胶作固定剂,将猪的味蕾组织固定到两片核微孔膜中间制成＂三明治＂式味觉传感膜,然后将其固定到玻碳电极上制成味觉生物传感电极,通过电化学工作站测定出蔗糖八乙酸酯、苯甲地那铵和槲皮素刺激其相应受体后的响应电流,结果表明：该传感器对蔗糖八乙酸酯、苯甲地那铵和槲皮素的最低检测限分别为1×10^-14、1×10^-13 mol/L和1×10^-14 mol/L,在浓度分别为1×10^-8、1×10^-6 mol/L和1×10^-9 mol/L时其电流变化率都相应的达到最大值,说明此时它们的受体已经被饱和。蔗糖八乙酸酯在其浓度为1×10-14~1×10^-11 mol/L有较好的对数关系（R2=0.957 3）,苯甲地那铵在其浓度为1×10^-13~1×10^-7 mol/L有很好的对数关系（R2=0.987 3）,而槲皮素在其浓度为1×10^-14~1×10^-9 mol/L时对数关系比较好（R2=0.996 4）。该传感器不仅可以定量化地测定味觉受体与苦味物质的作用,而且将为以后研究配体受体作用规律提供新的方法。

英文摘要：

Using sodium alginate-starch gel as a fixing agent,taste-bud tissues of pigs were fixed between two nuclear microporous membranes to make a sandwich-type sensing membrane,which was then fixed to a glassy carbon electrode to make a biosensor electrode.By using electrochemical workstation,the current produced when sucrose octaacetate,denatonium and quercetin stimulated their corresponding receptors were tested.The results showed that the lowest limit of detection of this biosensor for sucrose octaacetate,denatonium and quercetin were 1×10^-14,1×10^-13 and 1× 10^-14mol/L and the maximum rate of change of the current was found at concentration levels of 1×10^-8,1×10^-6and 1×10^-9mol/L,respectively,indicating that their receptors are saturated.It was demonstrated that the interaction curves of sucrose octaacetate,denatonium and quercetin with their respective receptors exhibited high correlation（R~2=0.957 3,0.987 3 and0.996 4） in the concentration ranges of 1×10^-14-1×10^-11mol/L,1×10^-13-1×10^-7mol/L and 1 × 10^-14-1×10^-9mol/L,respectively.This study not only quantitatively determined the interaction of the taste receptor and bitter substances with a new biosensor,but also provided a simple approach for monitoring bitter substances and investigating the mechanism of ligand-receptor interaction.