中文摘要：

用海藻酸钠-淀粉凝胶作固定剂,将SD大鼠的小肠组织固定到两片核微孔膜中间制成＂三明治＂式传感膜,然后将其固定到玻碳电极上制成生物传感电极,通过电化学工作站测定出不同质量浓度的人参皂苷刺激其相应受体后的响应电流。结果表明：在低质量浓度范围内,电流变化值呈线性增长,说明受体的量大于配基的量,后一部分呈曲线式增长,说明受体被逐渐饱和。用Origin 9软件对人参皂苷与其受体的作用曲线进行双曲线拟合（R2Adj=0.994）,可知小肠组织上存在人参皂苷受体,且其作用规律符合米氏酶促反应的双曲线模型;使用双倒数法（R2Adj=0.995）作图求出人参皂苷与其受体相互作用所产生的效应常数为2.969×10-16 g/m L。该传感器定量化地测定了人参皂苷通过其受体所发挥的生物学作用,这将为从肠道受体角度评价人参皂苷等生物活性成分的保健作用和功能评价提供新方法,为研究配体-受体作用规律提供新思路。

英文摘要：

Using sodium alginate-starch gel as a fixing agent, small intestine tissues from rats were fixed between two nuclear microporous membranes to make a sandwich-type membrane, which was then fixed onto a glassy carbon electrode to produce a biosensor electrode. The electrochemical workstation could test the current generated when ginsenoside stimulated the corresponding receptor. The results showed that in a low concentration range, the electric current value grew linearly, suggesting that the amount of receptor was greater than the amount of ligands. However, the final part of the growth curve exhibited a non-linear increase, suggesting that the receptors were gradually saturated. The action of ginsenoside and its receptor was fitted to a hyperbolic curve with Origin 9 software（R2Adj = 0.994）, which indicated the presence of ginsenoside receptors in the small intestine. The constant for ginsenoside and its receptor obtained with the double reciprocal method（R2Adj = 0.995） was 2.969 × 10-16 g/m L. This study has not only quantitatively determined the interaction of ginsenoside and its receptor substances with a new biosensor, but also has provided a simple approach for evaluating health benefits of ginseng saponins from the perspective of intestinal receptors and investigating the mechanism of ligand-receptor interaction.