中文摘要：

目的：探讨加味四逆散对异相睡眠剥夺168 h大鼠的学习记忆能力及前额叶皮质突触结构可塑性的作用。方法：以多平台法复制睡眠剥夺模型,药物干预组以加味四逆散灌胃,分别在睡眠剥夺6,30,54,78,102,126,150 h时间点给药,每次用药量为16.25 g.kg-1。应用Y迷宫评估动物学习与记忆能力的变化,并使用透射电镜观察动物前额叶皮质（prefrontalcortex,PFC）突触结构可塑性的改变。结果：加味四逆散组大鼠Y迷宫成绩明显高于模型组大鼠（P〈0.05）。与模型组比较,加味四逆散组大鼠PFC神经元突触间隙明显变窄（P〈0.05）,突触后膜致密物（post synaptic density,PSD）厚度明显变厚（P〈0.01）。结论：加味四逆散能改善异相睡眠剥夺模型大鼠学习与记忆能力,与其影响前额叶皮质神经元突触结构可塑性,从而增强其突触传递效能有关。

英文摘要：

Objective：To study the effects of Jiawei Sini powder on learning and memory ability and synaptic plasticity in prefrontal cortex of sleep deprived rats.Method：Experimental model of sleep deprivation was established by use of multi-platform method.The intervention group were fed with Jiawei Sini powder,and the dosage is 16.25 g·kg-1.The variation of learning and memory was detected by Y maze method at pre and post sleep deprivation.The changes of the ultrastructure of rat prefrontal cortex were observed by using transmission electron microscopy.Result：The percentage of correct responses of intervention group was significantly higher than those in model group（P0.05）.Compared with the model group,the post synaptic density（PSD） in prefrontal cortex areas in the intervention group was significantly thickened（P0.01）.Conclusion：Jiawei Sini powder could improve the ability of learning and memory in sleep deprived rats via increasing synaptic plasticity in prefrontal cortex.