中文摘要：

目的研究疏肝补肾方药对于疲劳大鼠海马CA1区钙/钙调蛋白依赖性激酶Ⅱ（CaMKⅡ）水平的变化。方法成年雄性Spargue-Dawley大鼠36只,随机分为模型组、对照组、疏肝补肾组。应用大鼠游泳运动结合睡眠剥夺建立疲劳大鼠复合模型,以Y迷宫实验评定其学习记忆能力。取大鼠海马CA1区以Western Blot定量检测,并以Real-time PCR技术分析海马CA1区CaMKⅡ的mRNA表达。结果 Y迷宫实验显示用药后大鼠的学习和记忆能力优于模型组,而疏肝补肾组大鼠在正确反应率和错误反应次数皆与模型组比较有统计学意义（P〈0.01）。Western Blot结果显示,模型组CaMKⅡ磷酸化的蛋白表达明显低于对照组（P〈0.01）,疏肝补肾组高于模型组（P〈0.01）。Real-time PCR结果显示,在mRNA水平上模型组及疏肝补肾组与对照组比较均有统计学意义（P〈0.01）,疏肝补肾组CaMKⅡmRNA表达高于模型组（P〈0.05）。结论疲劳引起大鼠海马CA1区的CaMKⅡ水平下调,而使用疏肝补肾方药可改善之,提示可由疏肝补肾法对抗疲劳引起的学习记忆损伤。

英文摘要：

Objective To study effect of regulating liver-Qi and tonifying kidney formula（RLTKF） on Ca/calmodulin dependent protein kinases Ⅱ（CaMKⅡ） level in the CA1 region of hippocampus in fatigue rats.Methods Thirty-six male adult Spargue-Dawley rats were randomly divided into three groups：Model group（n=12）,control group（n=12） and RLTKF group（n=12）.Fatigue models were established by exercise fatigue and sleep deprivation.Y maze was used to test the memory and learning of the rats.The CaMKⅡ expression in CA1 region of hippocampus was tested by western blot and real-time PCR.Results The ability of memory and learning in rats in RLTKF group was better than that in model group.The correct response rate,times of error reaction were significant difference between RLTKF group and model group（P0.01）.The expression of phosphorylated CaMKⅡ was lower in model group than that in control group（P0.01）.It was higher in RLTKF group than that in model group（P0.01）.There are also significant difference on CaMKⅡ mRNA expression in model group and RLTKF group with control group（P0.05）.Conclusion Fatigue could decrease the level of CaMKⅡ in the CA1 region of hippocampus,which could be ameliorated by RLTKF.The damage of learning and memory caused by fatigue could be antagonized through regulating liver-Qi and tonifying kidney therapy.