中文摘要：

目的：研究灯盏细辛中总咖啡酸酯（total Caffeoyl quinic acid ester，Caf）对离体培养的大鼠脑微血管内皮细胞（brain microvascular endothelial cell，BMEC）细胞间黏附分子-1（intercellular adhesion molecule-1，ICAM-1）基因与蛋白表达的影响，初步探讨caf抗脑缺血炎症损伤的机制。方法：采用肿瘤坏死因子-α（tumornecrosis factor-α，TNF-α）建立离体培养的大鼠BMEC炎症损伤模型，选用25μg／mL Caf处理内皮细胞，利用RealtimeRT-PCR方法检测实验各组ICAM-1mRNA表达，流式细胞仪测定实验各组ICAM-1蛋白荧光强度。结果：Caf可以下调TNF-α诱导的内皮细胞ICAM-1mRNA高表达，并抑制TNF-α诱导的内皮细胞膜ICAM-1蛋白高表达。结论：Caf可以降低由TNF-α诱导的内皮细胞过度表达ICAM-1，可能是其发挥抗脑缺血炎症损伤的部分机制。

英文摘要：

Objective：To investigate the effect of total Caffeoyl quinic acid ester（Car）on the expression of intercellular adhesion molecule-1 （ICAM-1）in rat brain miemvaseular endothelial cells（BMEC） cultivated in vitro. Methods：The inflammatory injury model was induced by tumor necrosis factor-α（TNF-α） in BMECs. The endothelial cells were treated with Caf at 25 μg/mL. The expression of ICAM-1 mRNA were detected by Real time RT-PCR,and ICAM-1 protein was measured by flow cytometry. Results： Car inhibited TNF-α-enhanced expression of ICAM-1 in BMECs. Conclusion ： Car decreases TNF-α-enhanced expression of ICAM- 1 in BMECs,which may be a protect effect on inflammatory injury after cerebral ischemia.