中文摘要：

目的观察清肠化湿方对实验性大鼠结肠炎结肠组织肿瘤坏死因子-α（TNF—α），紧密连接跨膜蛋白claudin-1表达水平的影响，以探讨其治疗溃疡性结肠炎（UC）的可能机制。方法使用TNBS／无水乙醇造模成功后，分为空白组、模型组、清肠化湿方组12．8g／（kg·d）、美沙拉嗪组（0．67g／（kg·d）。一次性遍给药，连续10d后，处死大鼠，留取结肠组织；ELISA法检测结肠组织TNF—α水平，免疫组化法观察结肠上皮claudin-1蛋白的表达情况，实时荧光定量PCR法检测claudin-1 mRNA相对表达水平。结果清肠化湿方可以降低大鼠结肠炎模型结肠组织TNF—α水平[模型组，清肠化湿组分别为（99．40±32．37），（55．07±12．80）pg／mL]，（P〈0．01），提高claudin-1mRNA相对表达水平[模型组，清肠化湿组分别为（2．18±0．78），（3．94±0．91）]（P〈0．01），减轻对claudin-1蛋白的损伤（P〈0．05），清肠化湿方组与美沙拉嗪组疗效无明显差异。结论清肠化湿方降低结肠组织TNF—α水平，保护紧密连接蛋白claudin-1，是其治疗UC的机制之一。

英文摘要：

OBJECTIVE To observe possible mechanism of the Qingehang Huashi Fang＇s therapeutic effect on Ulcerative Colitis（UC）, through ohserving the expression level of tumor necrosis faetor-α（TNF-α） and claudin-1 of mucosal tissue in the experimental colitis rats. METHODS Rats successfully modeled with TNBS/anhydrous alcohol were divided into blank, model, Qichang Huashi Fang（12.8 g crude drug/kg/d） and Mesalazine group（0.67 g/kg/d）. Single administration for 10 days successively, all groups of rats were executed to collect colon tissues cut out for related inspections. Using ELISA method to measure the TNF-α concentration of the colon tissues. The expression of claudin-1 protein in colonic epithelium was checked up with immunohistochemical method and relative expression of claudin-1 mRNA with real-time PCR method： RESULTS The TNF-α （pg/mL） concentration of model group and Qichang Huashi Fang group are respectively 99.40±32.37 and 55.07±12.80（P〈 0.01） ; And claudin-1 gene relative expression levels are respectively 2.18± 0.78 and 3.94 ±0.91 （P〈0.01） ; Qichang Huashi decoction can decrease TNF-αconcentration and increase claudin-1 mRNA relative expression significantly（P〈0.01）, thus reducing claudin-1 protein damage（P〈0.05）. The curative effect between Qichang Huashi Fang and Mesalazine group has no significant difference. CONCLUSION Qichang Huashi Fang can decrease TNF-α concentration and protect tight junction protein claudin-1. That is the one of the mechanisms of Qichang Huashi Fang to treat UC.