中文摘要：

目的 ：应用17β雌二醇（E2）作用于大鼠骨髓间充质干细胞,研究其对干细胞体外诱导成脂向分化的影响。方法：分离、培养原代大鼠骨髓间充质干细胞（rat bone mesenchymal stem cells,r BMSCs）,成脂诱导过程中加入10-9mol/L E2进行干预,采用油红O染色、实时荧光定量PCR、Western印迹等方法检测E2对干细胞脂向分化能力的影响。采用SPSS19.0软件包对数据进行统计学分析。结果 ：10-9 mol/L E2能显著抑制大鼠BMSCs内脂滴形成,并使成脂相关基因（C/EBPα、C/EBPβ、PPARγ、a P2及ARDP）表达显著降低,PPARγ及ARDP蛋白表达下降。结论 ：10-9 mol/L 17β雌二醇能显著抑制大鼠BMSCs成脂向分化。

英文摘要：

PURPOSE： In this study, 10-9 mol/L 17 β-estradiol （E2） was applied in the adipogenic differentiation of rat bone mesenchymal stem cells （rBMSCs） and the effect of E2 was explored. METHODS： Rat BMSCs were obtained from the femurs and tibias of SD rats. 10-9 mol/L E2 was involved in the adipogenic differentiation of rBMSCs. Oil red staining, real time PCR and Western blot were carried out to detect the effect of 10-9 mol/L E2 on adipogenie differentiation of rBMSCs. The data was statistically analyzed using SPSS 19.0 software package. RESULTS： The use of 10-9 mol/L E2 decreased the amount of lipid droplets in rBMSCs and weakened the expression of adipogenie related genes and proteins like C/EBP α, C/EBP β, PPAR γ, aP2, and ARDP, which were significantly lower than the adipogenic induced group. CONCLUSIONS： The use of 10-9 mol/L E2inhibited adipogenie differentiation of rBMSCs significantly in vitro. Supported by National Natural Science Foundation of China （81371121, 11342005, 30901698, 10972142）, Natural Science Foundation of Shanghai Municipality （13ZR1423700）, Key Basic Research Foundation of Science and Technology Committee of Shanghai Municipality （12JC1405700）, Collaborative Foundation of Medical and Engineering Science of Shanghai Jiao Tong University （YG2012MS40） and ＂Morning Star＂ Project from Shanghai Jiao Tong University.