中文摘要：

【目的】对侵染牛心柿引起炭疽病的病原菌进行分离鉴定，分析其致病性，初步构建其侵染谱，为柿树病害的病原诊断和防治提供理论依据和技术支持。【方法】通过组织分离法和单孢分离法从感病牛心柿的果实、叶片和嫩梢中分离病原菌，根据菌落和孢子形态特征及其 rDNA-ITS 序列分析进行种类鉴定，采用以菌饼进行伤口和非伤口接种、孢子悬浮液进行喷雾接种的离体接种试验分析其致病性。【结果】从感病牛心柿果实、叶片和嫩梢中分离获得9个病原菌分离物，菌落及孢子形态特征显示均为炭疽菌。以真菌转录间隔区通用引物 ITS6和 ITS4为引物，以菌丝总 DNA 为模板，扩增获得病原菌 rDNA-ITS基因片段，经 rDNA-ITS序列分析和系统进化树的构建，将该病原物鉴定为柿树炭疽菌，将其 rDNA-ITS 基因序列提交到 GenBank 数据库（基因登录号 KF010811）。牛心柿炭疽病菌和次郎炭疽病菌对不同品种柿树的致病性相同，均无差异性，对果实的致病性比叶片强；接种7天后，叶片无病斑或病斑较小，菌饼无伤口接种几乎不发病，在叶片上不产生病斑，果实上病斑较小；不同的接种方式在果实和叶片上产生的病斑大小为：菌饼伤口接种＞孢子悬浮液接种＞菌饼无伤口接种；牛心柿炭疽病菌和次郎炭疽菌对桃、苹果、梨等果树的叶片和果实均不致病。【结论】柿炭疽病菌具有一定的专性寄生性，对除柿树外的其他果树不致病，且在遗传上有一定的保守性，因地理距离产生的变异很小，这些特性对于柿炭疽病的防治具有重要意义，在一定程度上可以避免或削弱因病菌的遗传变异而产生的抗药性，从而有利于炭疽病的防治。在柿树有伤口时最易侵染致病，雨天或高湿度的情况下经孢子侵染致病的可能性居其次。在田间管理时注意避免造成柿树伤口感染，或?

英文摘要：

[Objective]This study aims to isolate and identify a pathogen which infects the persimmon plants and causes persimmon anthracnose,then investigate the pathogenicity so as to analyze its infect spectrum,and provide the theoretical basis and technical assistance for the pathogenic diagnosis and disease control of persimmon anthracnose in persimmon productivity.[Methods]The pathogen was isolated from anthracnose lesions on beef heart persimmon fruits,leaves and twigs in the persimmon orchards in Zhujiapo town,Linju county,Zhuge town,Yishui county and Wanjishan experimental station of Shandong Institute of Pomology with tissue separation and single spore separation, and identified by morphological characteristics and rDNA-ITS sequence analysis. The pathogenicity was investigated via in vitro inoculation test by disk-wound inoculation,disk-no-wound inoculation and spore-suspension inoculation.[Results]Nine isolates were obtained from persimmon fruits,leaves and twigs via tissue separation and single spore separation. The analysis of morphological characteristics showed that the 9 isolates belong to genus Colletotrichum. Then rDNA-ITS sequence analysis, via PCR amplification with the universal primers of ITS6 and ITS4 ,showed that the 9 isolates obtained were in full accord with the morphological observation,and the nucleotide sequence of rDNA-ITS gene （GenBank accession No. KF010811）shared 100%, 100% and 99. 8% identity with that of anthracnose pathogens on Jiro persimmon （ JQ957543 ） ,‘Wuheshi’persimmon in Zhejiang （ AY787483 ） and New Zealand isolate （ GQ329690 ） respectively. The phylogenetic analysis based on ITS sequences showed that beef heart persimmon isolate was located at the same branch of phylogenetic tree with the isolates of Jiro persimmon （JQ957543）,‘Wuheshi’persimmon in Zhejiang （AY787483,AY791890） and New Zealand persimmon （ GQ329687 , GQ329688 and GQ329690 ） , which indicated that the pathogen causing the anthracnose disease of beef heart persimmon could be identif