中文摘要：

目的构建PSGL-1＋/-；IL-2-/-小鼠模型。方法将白介素2敲除（IL-2KO）小鼠与P-选择素糖蛋白配体-1敲除（PSGL-1 KO）小鼠杂交，剪尾提DNA后用PCR法检测小鼠的基因型。结果IL-2KO小鼠与PSGL-1KO小鼠杂交三代后，PSGL-1＋/-；IL-2-/-小鼠模型构建成功。结论将IL-2KO小鼠与PSGL-1KO小鼠杂交三代后，成功建立了PSGL-1＋/-；IL-2-/-小鼠模型。

英文摘要：

Objective To construct the PSGL-1＋/-;IL-2-/- mice model. Methods PSGL-1 KO mice were mated with IL- 2 KO mice. Mice were genotyped by PCR after cut the tail and extracted DNA. Results After PSGL-1 KO mice being mated with IL-2 KO mice for three generations,PSGL-1＋/-;IL-2-/- mice were obtained. Conclusion PSGL-1＋/-;IL-2-/- mice are established through PSGL-1 KO mice being mated with IL-2 KO mice.