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中文摘要：
目的：建立可以快速检测人血浆中1-磷酸鞘氨醇（S1P）的LC-MS/MS定量分析方法。方法：采用C17-S1P作为内参,甲醇一步法进行蛋白沉淀,随后进行正相电喷雾离子化LC-MS/MS分析。流动相为甲醇-0.1%甲酸水（95∶5,v/v）,流速0.2ml/min,每个样品的分析时间为4min。结果：对于所有的质控样品,批内和批间精确度均小于12%。测定人血浆S1P的浓度为67.6ng/ml。结论：该方法可以快速、灵敏、特异性地检测血浆中S1P的含量。
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