对根霉产凝乳酶固体发酵条件进行研究。通过单因素分析和响应面试验设计,确定根霉产凝乳酶的发酵条件及发酵培养基的最佳组成为:24℃发酵7d;麸皮与江米质量比为0.96:1、培养基固液比(m/V)为0.99:1、奶粉添加质量分数为0.81%,所产的凝乳酶活力达到72.92SU/mL。
In this study,the solid-state fermentation conditions for producing chymosin with Rhizopus were optimized through single factor experiments and response surface methodology.The optimal fermentation conditions were media with solid-liquid ratio of 0.99:1(m/V),bran-rice ratio of 0.96:1(m/m),milk powder addition amount of 0.81%(m/m) and fermentation at 24 ℃ for 7 days.The activity of chymosin produced at this optimal condition reached up to 72.92 SU/mL.