中文摘要：

目的：研究肾移植患者中CYP3A4＊1G和CYP3A5＊3基因多态性对地尔硫？浓度/剂量比的影响。方法以47例肾移植患者为研究对象，用聚合酶链式反应-限制性内切片段长度多态性方法测定肾移植患者药物代谢酶基因型，用高效液相色谱法测定地尔硫？的血药浓度谷值，比较CYP3A4＊1G和CYP3A5＊3基因多态性对肾移植患者地尔硫？浓度/剂量比的影响。结果 CYP3 A4＊1＊1型14例（29.79％）、CYP3A4＊1＊1G型26例（55.32％），CYP3A4＊1G＊1G型7例（14.89％），G等位基因分布频率为57.45％，A等位基因分布频率为42.55％；CYP3A5＊1＊1型6例（12.77％）， CYP3A5＊1＊3型21例（44.68％）， CYP3A5＊3＊3型20例（42.55％），G等位基因分布频率为64.89％，A等位基因分布频率为35.11％。肾移植患者中CYP3A4＊1G基因多态性对地尔硫？浓度/剂量比有显著影响，CYP3A4＊1＊1型受者比携带CYP3A4＊1G等位基因肾移植患者的浓度/剂量比高1.52倍，差异有统计学意义（ P＜0.05）；而CYP3A5＊3基因多态性对浓度/剂量比影响较轻微，且差异无统计学意义。结论肾移植患者中CYP3A4＊1G基因多态性对地尔硫？浓度/剂量比有显著影响。

英文摘要：

Objective To investigate the impact of the CYP3A4＊1G and CYP3 A5＊3 polymorphisms on the concentration/dose ratios of dilti-azem in kidney transplant patients.Methods Forty -seven cases of kidney transplant recipients were recruited.CYP3A4 and CYP3A5 geno-types were determined by polymerase chain reaction -restriction fragment length polymorphism.Plasma trough concentration of diltiazem was meas-ured by HPLC.The effects of CYP3A4＊1G and CYP3A5＊3 gene poly-morphisms on the concentration/dose ratios of diltiazem were evaluated and compared.Results There were fourteen cases of CYP3A4＊1＊1 （29.79%） homozygous genotype , twenty six cases of CYP3A4＊1＊1G （55.32%） heterozygous genotype , and seven cases of CYP3A4＊1G＊1G （14.89%） homozygous genotype.Allele frequencies of CYP3A4＊1 and ＊1G were 57.45%and 42.55%, respectively.There were six ca-ses with CYP3A5＊1＊1 （12.77%）, twenty one cases with CYP3A5＊1＊3（44.68%）, and twenty cases with CYP3A5＊3＊3（42.55%）.Allele frequencies of CYP3A5＊1 and ＊3 were 35.11%and 64.89%,＆amp;nbsp;respectively.CYP3A4＊1G gene polymorphisms were significantly related to the concentration /dose of diltiazem in kidney transplant patients.The diltiazem blood trough concentration/dose in CYP3A4＊1＊1 recipients was 1.52 times higher than that in patients with CYP 3A4＊1G allelic genes.There was significant difference in diltiazem blood trough concen-tration/dose ratios between recipients with CYP3A4＊1＊1 genotypes and CYP3A4＊1G carriers（P＆lt;0.05）.CYP3A5＊3 gene polymorphisms had slight impacts on diltiazem blood trough concentration /dose ratios but no difference was found among the CYP3A5 genotypes.Conclusion The CYP3A4＊1G gene polymorphism is closely related to the blood con-centration/dose ratios of diltiazem in kidney transplant patients.