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中文摘要：
目的比较低分子量肝素和尿激酶对肾小球炎症反应的保护作用。方法3月龄雌性Wistar大鼠40只,随机分为对照组（NC组）、脂多糖（LPS）组（L组）、LPS＋氨甲环酸组（LT组）、LPS＋氨甲环酸＋低分子量肝素组（LTH组）及LPS＋氨甲环酸＋尿激酶组（LTU组）,每组8只。采用直接免疫荧光检测肾小球纤维蛋白沉积和CD11b阳性细胞分布情况,Western blotting检测内皮细胞细胞间黏附分子-1（ICAM-1）蛋白质表达。结果NC组大鼠肾小球内无纤维蛋白沉积和CD11b阳性细胞;ICAM-1有少量表达。与NC组比较,L组纤维蛋白沉积（9.1%±1.6%）和CD11b阳性细胞（11.2±2.1）增多（P〈0.05）;ICAM-1表达（0.23±0.09）明显上调（P〈0.05）。与L组比较,LT组纤维蛋白沉积（23.4%±3.2%）和CD11b阳性细胞（20.4±3.5）进一步增多;ICAM-1表达（0.44±0.16）进一步上调（P〈0.05）。与LT组比较,LTH组和LTU组纤维蛋白沉积（分别为11.3%±1.4%,10.6%±1.5%）和CD11b阳性细胞（分别为7.3±1.8,8.4±1.6）明显减少（P〈0.05或0.01）;ICAM-1表达（分别为0.19±0.10,0.18±0.09）明显下调（P〈0.05）,LTH、LTU两组间无统计学差异。结论低分子肝素与尿激酶均能有效减少纤维蛋白沉积,起到减轻肾小球炎症反应的作用。
英文摘要：
Objective To investigate the protective effects of low-molecular heparin and urokinase on glomerular inflammation. Methods Forty 3-month-old female Wistar rats were randomly divided into five groups with 8 rats in each group： normal control （NC） group; lipopolysaccharide （LPS） group; LPS and tranexamic acid （LT） group; LPS, tranexamic acid and low-molecular heparin （LTH） group; and LPS, tranexamic acid and urokinase （LTU） group. Fibrin deposition and CD11b positive cells were identified by immunohistochemical staining. Western blotting was used to determine the ICAM-1 expression. Results No fibrin depositions and CD11b positive cells were found in glomeruli of rats in NC group. Compared with NC group, fibrin deposit （9.1%±1.6%）, CD11b positive cell （11.2±2.1） and ICAM-1 expressions （0.23±0.09） were significantly increased in L group （P〈0.05）. Compared with L group, fibrin deposition （11.3%±1.4%, 10.6%±1.5%, respectively）, CD11b positive cells （7.3±1.8, 8.4±1.6, respectively） and ICAM-1 expressions （0.19±0.10, 0.18±0.09, respectively） were significant lower in LTH and LTU groups （P〈0.05）. No differences of fibrin deposition, CD11b positive cells and ICAM-1 expressions were found between LTH and LTU groups （P〉0.05）. Conclusion Both low-molecular heparin and urokinase can effectively decrease fibrin deposits and alleviate inflammation.
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