中文摘要：

在7颗新鲜拔除的离体人恒牙釉质光滑面制备2mm×3mm实验窗,选用pH4.5的化学酸蚀液在开窗区制备0,12,24,48,72,96,120h不同时间的早期脱矿人工龋模型,采用自行研发的口腔光学CT系统进行人恒牙早期牙釉质人工龋的体外检出实验研究,设立同一牙齿样本的临床显微摄影和扫描电镜观测对照,以扫描电镜超微组织学检出结果为金标准,评价光学CT体外检出牙釉质早期脱矿的效能。口腔光学CT系统可同时无创获得牙齿表面和截面的脱矿信息,准确检测到脱矿12h以内微米量级的早期牙釉质表面组织学水平的改变,早于裸眼观察和临床显微摄影。光学CT对于不同酸蚀期实验窗早期脱矿的检出与扫描电镜表征完全一致。口腔光学CT可以牙齿表面快速扫描的方式,非接触、准确、量化地检出牙釉质早期人工脱矿,检出敏感度高,重复性好,可无创获得脱矿部位微米量级量化分析数据。

英文摘要：

2mm×3mm experimental windows were prepared in adamant slippery surfaces of 7 fresh uprooted permanent teeth. The teeth surfaces in the windows area were demineralized to create artificial caries mould of early stage by aciding the experimental teeth surfaces of 0, 12, 24, 48, 72, 96, 120 hours with demineralized liquid （pH 4.5） in vitro. The demineralized changes on the experimental teeth surfaces were detected by dental Optical Coherence Tomography （OCT） system, which were newly developed by our research group recently, and the detecting results were compared with clinical digital photomicrography and scanning electron microscopy on the same tooth sample, to checkout the efficacy and feasibility of dental OCT for early quantification detecting of artificial enamel demineralization in vitro. The dental OCT system can safely detect early enamel demineralization of micron level and noninvasively obtain fine resolution quantification information both in surfaces view and sectional view; OCT could accurately detect surface demineralization changes on the experimental windows of artificial dental caries as early as after 12 hours aciding treatment, earlier than the visual inspection and clinical digital photomicrography. OCT could obtain both superficial view and sectional view of quantificational demineralization in early enamel caries homecchronously, and had high correlation to the results of ultramicromorphological changes detected by scanning electron microscopy. Dental OCT system developed by our group could accurately detect early artificial dental caries atraumaticly with high sensitivity and safety. Moreover, it can obtain quantification data in micron level without damaging the experimental teeth samples.