中文摘要：

运用Bioedit软件,从已知耐热性能较好的G/11木聚糖酶蛋白序列出发,构建调和序列,获得新型木聚糖酶.首先运用InterProScan得出该序列为G/11家族,再运用MotifScan确定其活性部位,对比G/11木聚糖酶的特征序列确定该酶为木聚糖酶.通过SWISS-MODEL对其三级结构进行预测,了解信号肽长度为27个.运用Protparam Tool对其部分理化性质进行预测,得出其理论等电点为6.82,最适温度为81.72℃,高于调和前的任何一条序列.最后,通过选择宿主菌最优密码子获得其基因序列.

英文摘要：

A novel xylanase sequence was obtained by using 13ioedit to compare six G/11 thermostable xylanase protein sequences. The calculated optimal temperature of the new sequence was 81.72 ℃, which was higher than any of the six ancestral sequences. Firstly, we knew this new sequence belonged to G/11 through using InterProScan, and got its active site by using MotifScan. The signal peptide of the new xylanase could know by using the SWISS-MODEL which predicted the tertiary structure of the protein, The length of the signal peptide was 27. By using protparam tool, we got some physical and chemical properties of the new xylanase, from which we knew the theoretical isoelectric point of it was 6. 28. The gene encoding of the xylanase was generated through the optimal codon used by the host strain. The current study paved the way for the synthetic biology of xylanase.