中文摘要：

将采集于清源山、华大后山果园、华大南浦果园、实验室附近等多处的高温堆肥样品在60℃、pH7．0条件下进行富集培养，其中南浦果园堆肥样品菌体长势较好，菌株呈暗绿色；逐步提高培养基中甘油浓度（0．5g/L-3g／L），进行菌株产甘油脱氢酶（GDH）的驯化培养与复筛，经8个批次的复筛，获得一株产GDH酶活达0．201U／mL的棕色菌株，编号TB8-5；经镜检分析同时结合菌落生长特性并经生理生化实验反应鉴定，初步判断筛选出的菌株属嗜热芽孢杆菌属；菌株发酵液中产物组分鉴定为二羟基丙酮（DHA），含量为0.597mg／mL，相应的GDH酶活也最高，其最适反应温度为50℃，最适反应条件pH为8．0，Zn^2＋对酶活有一定的促进作用。

英文摘要：

Samples of high temperature compost collected from different point（named QYS, HS, NP, ZZ） were enrichment cultured at 60℃, pH7.0. Bacterium growed preferable was obtained from sample of NP and submit sap green; The strain was domesticationed for produce Glycerol Dehydrogenase （GDH） by improved glycerol from 0.5g/L to 3g/L and a Strain that produced GDH with 0.2 U/mL activity was acquired after 8 batchs screened, numbered TB8-5 and distinguished as Therrnophili Bacillus; Produce in ferment liquid was identified as Dihydroxyacetone （DHA） and it,s most content was 0.597 mg/mL, relevant GDH activity also highest and optimum temperature of the GDH was 50℃, optimum pH was 8.0, Zn^2＋ facilitated to enzyme activity.