中文摘要：

目的考察（S,S）ZX-5对eNOS mRNA转录与eNOS蛋白表达的影响,从分子水平探讨（S,S）ZX-5促进一氧化氮（NO）释放的作用机制。方法用不同浓度的新型化合物（S,S）ZX-5刺激ECV30424h,采用RT-PCR半定量方法研究（S,S）ZX-5对ECV304eNOS的mRNA转录水平的影响;用Westernblot方法研究（S,S）ZX-5对ECV304eNOS蛋白的表达量变化影响。结果30mg·L^-1（S,S）ZX-5使ECV304的eNOS mRNA转录量增加119%、使eNOS蛋白表达量增加62%;30mg·L^-1（R,R）ZX-5的上述作用不显著。结论（S,S）ZX-5促进ECV304NO释放的作用机制是通过增加内皮型的NOS（eNOS）mRNA转录,提高eNOS蛋白的表达水平,从而提高了eNOS酶的活性。该研究为将（S,S）ZX-5开发为通过增加NO释放从而选择性改善脉络膜血流的药物提供实验依据。

英文摘要：

Aim To investigate the effects of（S,S）ZX-5 and（R,R）ZX-5 on eNOS mRNA and protein levels.Methods Cell culture was used for the experiments in vitro.The effects of eNOS mRNA were investigated by RT-PCR.The effects of eNOS protein levels were investigated by Western blot.Results（S,S）ZX-5 in concentration of 30 mg·L^-1 up-regulated eNOS expressions at protein and mRNA levels significantly in cultured ECV304（P〈0.01）.The effects of（R,R）ZX-5 was of no significant difference.Conclusion The mechanism of NO increases in ECV304 by（S,S）ZX-5 were up-regulation of eNOS expressions at protein and mRNA levels.The result provide experimental evidence for a new drug for prevention and treatment of AMD（age-related macular degeneration）.