中文摘要：

目的：建立单倍型相合小鼠急性移植物抗宿主病（aGVHD）模型,探讨移植后继发血细胞减少的原因。方法：将受鼠分为单纯照射（R组）、照射＋输注骨髓单个核细胞（B组）、照射＋输骨髓单个核细胞＋输脾单个核细胞（S组）。移植后观察小鼠体重、体位、活性、毛发、腹泻及生存期,检测外周血象及嵌合体,濒死前行肝、小肠、皮肤、脾脏病理检查。结果：S组出现体重降低、弓背、活动度减低、脱毛,病理检查符合aGVHD,并发生继发白细胞及血红蛋白降低,同时检测嵌合体〉90%。结论：C57BL/6N→BCF1 9.5 Gy^60Co的照射剂量下,输注骨髓单个核细胞1×10^7,脾单个核细胞3×10^7、4×10^7、5×10^7均可建立aGVHD模型;aGVHD的发生是移植后继发血细胞减少的主要原因。

英文摘要：

Objective： To find acute graft-versus-host disease model in murine haploidentical stem cell transplantation and to explore secondary cytopenia after transplantation.Methods： C57BL/6N mice were donor,BCF1 mice（C57BL/6N×BALB/c） were recipient.Recipient mice were divided into irradiation group（R）、 irradiation add inoculating bone marrow cell group（B）、 irradiation add inoculating bone marrow cell add splenocyte group（S）.After transplantation,mice were checked daily for the appearance of aGVHD symptoms such as hunched posture,ruffled fur,diarrhea,and survival.Blood routine test was done twice weekly,chimerism was detected by FACS,and Liver,skin,intestine,and spleen were biopsied for histopathological examination.Results： S group showed reduction of weight,hunched posture,ruffled fur and death.aGVHD was verified by histopathological examination.Secondary cytopenia after transplantation occurred in S group,while chimerism was more than 90% at the same time.Conclusion： aGVHD model in murine haploidentical stem cell transplantation can be founded when irradiation dose of 9.5 Gy,inoculated with 10^7 bone marrow cell and 3×10^7 or 4×10^7 or 5×10^7 splenocyte,and aGVHD was main reason of Secondary cytopenia in S group.