中文摘要：

实验通过对兔子基因组DNA进行PCR获得兔IFRG基因,将其克隆到pGEM-T载体,双酶切鉴定和测序结果表明成功构建了重组克隆载体pGEM-T-IFRG。通过RT-PCR分析发现IFRG基因在兔不同组织中有不同程度的表达。将重组克隆载体pGEM-T-IFRG和表达载体pET-41（c）经过EcoR1和Xho1双酶切后连接构建重组表达载体pET-41（c）-IFRG,将双酶切鉴定正确的重组表达载体转化入E.coli BL21,IPTG诱导融合蛋白的表达,SDS-PAGE结果显示兔IFRG基因在大肠杆菌中得到了良好的表达。

英文摘要：

The rabbit IFRG gene was gained by PCR from the rabbit genomic DNA and the gene was cloned into pGEM-T vector.The double enzymes digestion and sequence analysis showed that the construction of recombinant vector pGEM-T-IFRG was successful.RT-PCR analysis of rabbit different organism showed that IFRG gene expressed distinct in different organism.To construct recombinant expression vector pET-41（c）-IFRG by the double enzymes digestion of recombinant pGEM-T-IFRG and pET-41（c）,the correct recombinant expression vector that identified with double enzymes digestion was transformed into E.coli BL21（DE3）.Fusional protein was expressed under IPTG induction.SDS-PAGE showed that the rabbit IFRG gene had a favourable expression in bacterium.