中文摘要：

目的通过观察去卵巢骨质疏松症模型大鼠股骨中Smurf1的表达变化，探索骨质疏松症的发病机理。方法实验采用切除大鼠双侧卵巢的方法复制骨质疏松症模型，同时应用补肾填精作用的纳米钙补肾填精中药低、高剂量组，对模型大鼠给药干预12周，用骨疏康颗粒剂、盖天力作为阳性对照组，以及正常对照组、假手术对照组和模型空白组，并与补脾中药补中益气汤作比较；免疫组化SABC法检测股骨中的Smurf1蛋白活性表达。结果与正常组相比较，模空组Smurf1阳性表达显著下降，P〈0．01；用药12周后，与模空组相比较，各用药组均可上调其表达情况，P〈0．01。结论骨组织中Smurf1阳性表达降低可能是骨质疏松症发生的机制之一，补肾填精中药可能通过改善和调控股骨中Smurf1的表达，而起到防治骨质疏松症的作用。

英文摘要：

Objective To explore the pathogenesis of osteoporosis by observation of the alteration of Smurf1 expression in rat femurs of ovarieetomized osteoporosis model. Methods The osteoporosis rat model was established using ovriectomy method. The Nano-calcium Chinese medicine that has invigorating kidney for consolidation semen function （high and low dose） was administered to modeled rats for 12 weeks. Gushukang granule and Gaitianli tablets groups were as positive control. Normal control group, sham operation group, and model control group were used. Buzhong yiqi decoction to invigorating spleen group was for comparison. The expression of Smurf1 proteins was measured using IHC method. Results Smurf1 protein positive expression in rats of model group decreased significantly, compared to that of normal control group （P 〈 0. 01 ）. Smurf1 protein expression in all dose groups increased, compared to that of the model control group after a 12-week drug use （P 〈 0. 01 ）. Conclusion The decreasing of Smurf1 protein expression in the bone tissue may be one of the mechanisms of osteoporosis pathogenesis. The invigorating kidney for consolidation semen Chinese medicine may prevent and treat osteoporosis by improving and regulating the expression of Smurf1 protein.