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中文摘要：
以T–DNA插入引起的水稻矮秆多分蘖突变体丛矮648（CA648）及对照中花11（ZH11）为材料,进行农艺性状、F2杂交群体性状分离分析及分子验证。结果显示：CA648表现出矮化、多分蘖、花期延迟等突变性状;CA648与ZH11杂交后的F2群体的不同株高统计结果符合单基因控制的1∶3遗传分离比例,F2代材料中所有抗Basta株系均表现出矮化多分蘖特性,且非抗性株系与抗性株系数量之比符合1∶3分离规律;TAIL–PCR等分子试验结合生物信息学分析结果表明,T–DNA插在8#染色体一个功能未知基因LOC_Os08g34258的内部,该基因编码蛋白可能与Subtilisin inhibitor家族蛋白具有类似功能。
英文摘要：
The rice mutant（CA648） with a T-DNA insertion and a wild-type rice of Zhonghua 11（ZH11） were used to analyze the agronomic and molecular traits in this paper. Compared with ZH11, CA648 displayed a phenotype of dwarfism, multi-tillering and late flowering. In the F2 population, the ratio of thenormal plants to the semi-dwarfing or dwarfing plants was approximately 1∶3. All of the plants resistant to Basta in F2 population were short and multi-tillering. Furthermore, the ratio of non-resistant strains to resistant strains in F2 population is 1∶3. Thermal asymmetric interlaced（TAIL）-PCR analysis and bioinformatics analysis showed that the T-DNA was inserted in the 8thchromosome of an function unknown gene LOC_Os08g34258, which encodes a protein with the function similar to proteins of the subtilisin inhibitor family.
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