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中文摘要：
传统IAA测定方法难以满足目前相关研究对生长素测定提出的新要求，建立符合目前植物激素前沿研究需求的单细胞水平微量生长素原位实时测定新方法已成为当务之急。本项目拟在前期研究工作基础上开展以下研究进一步利用转DR5::GFP的拟南芥IAA低水平合成双突变体yuc2yuc6为研究材料，基于GFP的荧光强度、分子数量和mRNA表达量等多条途径建立IAA浓度与GFP表达量之间的准确数量关系，建立IAA高灵敏原位实时测定方法；在小立碗藓中构建双元（生长素和温度）诱导的可控GFP表达系统，通过与IAA诱导的DII-GFP降解系统进行对比，建立单细胞水平的IAA准确定量方法；基于IAA共受体和二维液相建立微量样品中IAA的高效分离纯化新技术；基于DNA末端保护小分子分析技术建立IAA的高灵敏检测新方法；系统开展所建立新方法的应用技术研究和检测应用示范，以促进我国植物激素检测的技术进步。
结论摘要：
英文主题词IAA；Single cell；In-situ；Assay；
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