中文摘要：

目的：研究体外脐血造血干/祖细胞向B细胞分化的条件。方法：体外免疫磁珠分离纯化脐血CD34^＋CD19^-造血干/祖细胞;在小鼠S-17基质细胞支持下,脐血CD34^＋CD19^-造血干/祖细胞、T3、各种细胞因子共培养建立体外B细胞分化发育培养体系,诱导脐血CD34^＋CD19^-造血干/祖细胞向B细胞分化;用流式细胞仪检测培养的B细胞。结果：T3、IL-7与小鼠S-17基质细胞共培养诱导CD34^＋CD19^-造血干/祖细胞28天时,分化形成的B细胞数可达初始培养细胞的198倍,诱导细胞大部分表达CD10、CD19。结论：在选用的实验条件下,T3、IL-7与小鼠S-17基质细胞体外能诱导脐血造血干/祖细胞的B细胞分化。

英文摘要：

Objective：To investigate the condition of B cell differentiation from cord blood CD34^＋ CD19^- hematopoietic stem/progenitor cells in vitro. Methods： CD34^＋ CD19^- cells from cord blood were isolated and purified by using immunomagnetic beads separation system. CD34 ^＋ CD19^- cells supported by murine S-17 stromal cells were stimulated in co-culture with T3 and cytokines. Differentiated B cells were analyzed by flow cytometry. Results： Tne amplification of the B cells derived from CD34 ^＋ CD19^- hematopoietic stem/progenitor cells in co-culture with T3 and IL-7 reached 198-fold of increase, most of the induced cells expressed CD10 and CD19. Conclusion： In the experimental conditions selected, co-culture of CD34^＋ CD19^- cells with T3, IL-7 and murine S-17 stromal cells could stimulate differentiate toward to B cells in vitro.