中文摘要：

目的观察电刺激海马CA1区对下丘脑室旁核（PVN）胃牵张（GD）敏感神经元放电活动的影响,以及ghrelin在该通路中的调控作用。方法采用细胞外记录神经元单位放电方法,观察电刺激海马CA1区、ghrelin及其受体阻断剂[D-Lys-3]-GHRP-6对大鼠下丘脑PVN内GD敏感神经元放电活动的影响。结果在PVN记录到的109个GD敏感神经元中,有71个为GD兴奋性（GD-E）神经元,38个为GD抑制性（GD-I）神经元。在GD-E神经元中,微量注射ghrelin可兴奋其中72%的神经元,放电频率增加（38.9±7.3）%（t=2.85,P〈0.01）;而在GD-I神经元中,微量注射ghrelin可抑制其中60%的神经元,放电频率减少（45.2±6.3）%（t=3.08,P〈0.01）;gh-relin的效应可被[D-Lys-3]-GHRP-6阻断。在41个对ghrelin有兴奋反应的GD-E神经元和15个对ghrelin有抑制反应的GD-I神经元中,电刺激海马CA1区,可分别兴奋39%的GD-E和33%的GD-I神经元,其中44%的GD-E神经元的兴奋效应可被[D-Lys-3]-GHRP-6部分阻断。结论海马CA1区可以调控PVN内GD敏感神经元的活性,ghrelin能神经纤维参与了该通路的调控。

英文摘要：

Objective To explore the effect of electric stimulation of CA1 area on the activity of gastric distention（GD） sensitive neurons in PVN and the role of ghrelin in this nerve pathway.Methods The effects of ghrelin on GD sensitive neurons in PVN and the effects of electric stimulation of CA1 area on the activity of these neurons were observed by recording extracellular potentials of single neurons.The effects of antagonist of ghrelin-[D-Lys-3]-GHRP-6 were also observed to explore the receptor involved.Results In 109 GD sensitivity neurons recorded by the PVN,71 were classified as GD-excitatory（GD-E） neurons,and 38 were GD-inhibitory（GD-I）.Microinjection of ghrelin excited 72% GD-E neurons,and discharge frequency increased（38.9±7.3）%（t=2.85,P〈0.01）;in GD-I neurons,microinjection of ghrelin could inhibit 60% of the neurons,and discharge frequency decreased（45.2±6.3）%（t=3.08,P〈0.01）.The effect of ghrelin could be blocked by [D-Lys-3]-GHRP-6.Among 41 GD-E neurons excited by ghrelin and 15 GD-I neurons inhibited by ghrelin,electric stimulation of CA1 area of hippocampus could stimulate 39% of GD-E neurons and 33% of GD-I neurons,respectively,in which,44% of excitement effect of GD-E could be partially blocked by [D-Lys-3]-GHRP-6.Conclusion The neurons of the hippocampus CA1 area can regulate the activity of GD sensitive neurons in PVN and this effect is mediated by ghrelin-energy fibers.