中文摘要：

目的：分离纯化双歧杆菌细胞壁中的完整肽聚糖（whole peptidoglycan．WPG）．并制备靶向抗癌药物即完整肽聚糖白蛋白磁性微球。方法：利用复合酶法提取双歧杆菌细胞壁中完整肽聚糖．以人血清白蛋白（HSA）和纳采Fe2O3为载体．采用乳化高温固化法制备出包含有完整肽聚糖的白蛋白磁性微球，并利用扫描电镜对微观形态进行观察，同时采用紫外分光光度计法对其载药量进行检测。结果：制备出高纯度的完整肽聚糖。磁性微球在电镜下呈表面光滑的核-壳样球型微粒．结合后的平均载药量和包封率分别为5．53％和27．5％。结论：利用乳化高温固化法可以制备出完整肽聚糖白蛋白磁性微球．并为肿瘤的主动靶向治疗提供了一种可能的新剂型。

英文摘要：

OBJECFIVE To isolate and purify whole peptidoglycan from cell wall of bifidobacterium bifidum,and to prepare anti-cancer drug the whole peptidoglycan albumin magnetic microspheres. METHODS Whole peptidoglycan was extracted from cell wall of bifidobactcrium bifidum by the method of compound enzymes, then it was solidified with human serum albu min（HSA） and nano-Fe3O4 at high temperature to prepare the whole peptidoglycan albumin magnetic microspheres. The microcosmic configuration of microspheres was detected by scanning electron microscope, and the amount of drug carried was also assayed by ultraviolet spectrophotometry. RESULTS The purity of whole peptidoglycan prepared was very high. Under the e lectron microscopy the magnetic microsphere ,showed a smooth spheroidal particle with core shell, and the drug loading and em bedding ratios were 5.53% and 27.5%, respectively. CONCLUSION The whole peptidoglycan albumin magnetic micro spheres could be made by the method of emulsification and solidification, and may offer a novel approach to target the therapeu tic basis of tumors.